脏腑别通理论的源流和机理及其应用  

Effect of Modified Sini Powder on nNOS Expression in Hippocampus of Rats with Psychological-Stress-Induced Damage

作　　者：王旭[1] 吴爱华[1] 刘雁[1] 

机构地区：[1]山东菏泽医学专科学校,山东菏泽274000

出　　处：《广州中医药大学学报》2007年第5期427-429,共3页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：回顾《医学入门》及其之后关于脏腑别通理论的文献记载,探讨关于其机理的不同学说,认为三阴三阳开阖枢学说为其理论基础。指出脏腑别通理论从多方面拓展了藏象学说中脏与腑之间的联系,具有理论和临床上的现实意义,突出表现为脏腑别通理论在针灸腧穴学上的应用,为腧穴学提出了一种新的配穴法。【Objective】To observe the effect of Modified Sini Powder(MSP)on the expression of hippocampal neuronal nitric oxide synthase(nNOS)in rats with chronic psychological stress.【Methods】Twenty-four Wistar rats were randomized into 3 groups: normal group,model group(receiving random stress stimulation for 21 days),and MSP group(receiving gastric infusion of MSP 3.38 g for each rat one hour before stimulation).The normal group and the model group received the same volume of saline.The expression of hippocampal nNOS was detected with immunohistochemical method.【Results】nNOS expression was found in neuronal pyramidal cells of hippocampus CA3 area in the three groups.The expression was obvious,positive cell count and its scoring were increased in the model group as compared with the normal group(P<0.01),and MSP reduced the nNOS expression as well as positive cell count and its scoring in rats with chronic stress(P<0.01).【Conclusion】MSP can relieve the damage induced by chronic stress in rats,and its mechanism is probably related with the high-expression of nNOS in hippocampus,thus avoid the increase of NO damaging neuronal cells.

关 键 词：脏腑经络关系 脏腑别通 开阖补泻 配穴方法 

分 类 号：R223.1[医药卫生—中医基础理论]