亲水性单胞菌几丁质酶发酵工艺优化  

Optimization of Fermentation Technology for Chitinase Production by Aeromonas hydrophila sp.

作　　者：吕美巧[1] 段文凯[1] 姚祥春[1] 张礼星[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310014

出　　处：《科技通报》2007年第5期676-680,共5页Bulletin of Science and Technology

基　　金：浙江省温州市和平阳县科技局资助

摘　　要：首次报道从温州浅海海水中筛选得到的一株产几丁质酶的亲水性单胞菌属细菌(Aeromonas hydrophila sp.)。在探索了碳、氮源和环境因子对细菌产酶影响的基础上,利用均匀设计法优化得到了较佳的产酶发酵培养基配方(g/L):胶体几丁质5.0,蛋白胨12.0,尿素2.0,MgSO4 0.5,K2HPO4 0.3,NaCl5.0,初始pH 6.5。在接种量6%,培养温度28℃,180 r/min的较佳工艺下振荡发酵5 d,该菌产酶活力达到1.89 U/mL。It was the first time to report a chitinase-producing marine strain Aeromonas hydrophila sp. isolated from Wenzhou neritie seawaters. Firstly the effects of carbon and nitrogen source and environmental factors on the production of ehitinase were studied. Further more uniform-design method was used to get an optimal fermentation medium containing （g/L） colloid chitin 5.0, peptone 12.0, urea 2.0, MgSO4 0.5, K2HPO4 0.3, NaC1 5.0, pH6.5. The enzyme was produced under optimal conditions of fermentation time （5 d） in the medium with inoculum size （6% v/v） at 28℃ andl80 r/min shaking, and showed a maximum enzyme activity of 1.89U/mL.

关 键 词：亲水性单胞菌 几丁质酶 发酵 

分 类 号：Q814[生物学—生物工程]