FISH/MAR技术及其在环境微生物学研究中的应用  

FISH/MAR Technique and Its Application in Study of Environmental Microbiology

作　　者：张瑛[1] 阮晓红[1] 

机构地区：[1]河海大学水文水资源与水利工程科学国家重点实验室

出　　处：《环境科学与技术》2007年第1期100-104,共5页Environmental Science & Technology

基　　金：国家"十五"重大科技专项基金资助项目(2003AA601070);国家自然科学基金(40573051)

摘　　要：荧光原位杂交与微量自动照相集成技术(FISH/MAR)能够同时在单细胞水平原位进行细胞的分类鉴定和细胞对底物的吸收特性研究,这是微生物学研究领域的一大突破。目前,这一技术已有效应用于工程系统(污水处理厂、生物反应器等)和自然生态系统中微生物的研究。污染水体的原位强化净化及其生态修复与微生物也有着密切的关系,因此,这一技术的应用不仅能够提高工程系统污水处理效率,也将为受损水生态系统的原位修复提供科学依据。Combined technique of fluorescence in situ hybridisation and microautoradiography (FISH/MAR) can be used to study the identity of microorganism and their key physiological features simultaneously. The application of FISH/MAR to the study of microorganism in structure system including wastewater treatment plant and bioreactor and natural ecosystem was discussed. Since on site treatment of polluted waters was also closely related to the function of microorganisms, this technique not only can improve wastewater treatment efficiency in structural system but also provide scientific basis for rehabilitation of degraded aquatic ecosystems.

关 键 词：荧光原位杂交(FISH) 微量自动照相术(MAR) 微生物 种属特性 功能特性 

分 类 号：X172[环境科学与工程—环境科学]