白细胞介素1β介导小型猪冠状动脉粥样硬化病变与药物诱发冠状动脉痉挛模型的研究  

Development of Coronary Artery Atherosclerosis and Coronary Artery Spasm Model in Chinese Mini-swine Mediated by Interleukin-1β

作　　者：关启刚[1] 程颖[1] 曾定尹[1] 孙喜琢[2] 何学志[2] 韩凤桐[2] 周旭晨[1] 苗志林[1] 张利[2] 

机构地区：[1]中国医科大学附属第一医院心内科,辽宁省沈阳市110001 [2]大连市中心医院心内科,辽宁省大连市116033

出　　处：《中国动脉硬化杂志》2007年第4期241-244,共4页Chinese Journal of Arteriosclerosis

基　　金：国家重点基础研究发展计划(973计划)资助项目(2005CB523310)

摘　　要：目的探讨白细胞介素1β介导的小型猪冠状动脉粥样硬化病变与药物诱发冠状动脉痉挛模型的构建方法。方法小型雄性家猪随机分为3组,每组8头,开胸手术分离冠状动脉左前降支和回旋支近端,对照组血管外膜包裹吸附含生理盐水琼脂糖微粒悬液的纸巾;模型1组和模型2组分别包裹吸附含白细胞介素1β2.5μg和6.0μg琼脂糖微粒悬液的纸巾。2周后冠状动脉造影观察管腔狭窄程度及分别往冠状动脉内注5羟色胺(3μg/kg,10μg/kg)和组胺(3μg/kg,10μg/kg)诱发冠状动脉痉挛情况。光镜观察管腔病理学改变。结果冠状动脉外膜包裹白细胞介素1β血管段发生不同程度的管腔狭窄,5羟色胺和组胺均可诱发模型1组(冠状动脉狭窄20%～30%)病变血管段痉挛,阳性率为82%,与对照组比较差异有显著性(P<0.05)。而模型2组(冠状动脉狭窄50%～70%)均未诱发出痉挛。两组光镜均可见病变血管段内膜增殖和炎症细胞聚集现象。结论白细胞介素1β包裹冠状动脉外膜可引起动脉粥样硬化病变,5羟色胺和组胺可诱发轻度狭窄的冠状动脉发生痉挛。Aim To study development coronary artery Atherosclerosis and coronary artery spasm model in Chinese mini-swine mediated by inflammatory factor. Methods Chinese mini-swine were randomly divided into three groups. For establishing coronary artery atherosclerosis and coronary artery spasm model, chest operation was done by isolation of left-anterior descending banch and circumflex branch proximate. Coronary artery membranna extema in swine was packed by paper towel absorbing saline ngarose particle suspension in control group. Coronary artery membranna extema in swine was packed by paper towel absorbing IL-1β （2.5μg） agarose particle suspension in model 1 group. Coronary artery membranna extema was packed by paper towel absorbing IL-1β （6.0μg）agarose particle suspension in model 2 group. After 2 weeks the state of coronary artery spasm wass induced by injecting 5-serotonin （3 μg/kg, 10 μg/kg） and histamine （3 μg/kg, 10μg/kg）into coronary artery via conduct. I.amens narrow was observed by coronary arteriogramphy. Results Results narrow and CAS in model 1 group were observed. Positive rate of coronary artery lumens narrow was 20 ～ 30%, positive rate of CAS was 82 %. CAS in model 2 group was not observed. The pathological examination showed that the macrophage accumulation could be found at different degrees in both groups. Conclusions 5-Hydroxytryptamine and histamine can induce CAS, Atherosclerosis and lumens narrow produce in coronary artery membrana extema packed by IL-1β.

关 键 词：病理学与病理生理学 动脉粥样硬化 冠状血管痉挛 白细胞介素1 5羟色胺 组胺 

分 类 号：R363[医药卫生—病理学]