热带假丝酵母8953菌株对苯酚的降解特性研究  

Characteristics of Candida Tropicalis for Phenol Degradation

作　　者：梁树才[1] 杨宝玉[1] 刘海舟[1] 张勇[1] 陈士云[1] 

机构地区：[1]中国科学院武汉病毒研究所

出　　处：《环境科学与技术》2007年第3期27-28,31,共3页Environmental Science & Technology

基　　金：湖北省科技攻关资助项目(2004AA305B02);湖北省青年杰出人才基金资助(2003AB016)

摘　　要：实验室分离的一株热带假丝酵母(编号为8953)具有较强的苯酚降解能力,并可以利用苯酚、间苯二酚、联苯胺等芳烃为唯一碳源和能源生长。该菌株在48h内可完全降解约14.88mmol/L苯酚,完全降解10.63mmol/L苯酚只需要24h,其降解苯酚浓度最高可达19.13mmol/L。在pH4～9,温度20℃～40℃范围内,苯酚浓度为10.0mmol/L条件下该菌株均保持100%的降解率。该菌株降解苯酚的最适pH范围为6.0～8.0,最适温度范围为28℃～30℃,最佳接种量为1%～3%,外源添加氮源能促进酵母生长和苯酚的降解。A yeast strain was isolated from sesame flower and classified as Candida tropicalis, with phenol, resorcin and bengidine as the sole carbon and energy resource. The strain can degrade phenol up to 14.88mmol/L within 48h and the highest phenol concentration to be degraded was 19.13mmol/L. Degradation efficiency of 100% was obtained when the strain was cultivated in NMS medium containing 10.0mmol/L phenol with pH ranging from 4.0 to 9.0 and at temperature ranging from 20℃ to 40℃. The optimum culture conditions for phenol degradation were pH 6.0~8.0, 28℃~30℃, with inoculation ratio ranging from 1% to 3%. Growth and phenol degradation was enhanced when additional nitrogen resource was added into culture medium.

关 键 词：苯酚 生物降解 热带假丝酵母 

分 类 号：X172[环境科学与工程—环境科学]