人胃癌组织中DNA聚合酶β基因突变的研究  

Study on DNA polymerase β gene mutation in human gastric cancer tissues

作　　者：赵洁 米文 薛书尊 董子明[2] 沈平 赵国强[2] 

机构地区：[1]济南军区第155中心医院消化内科,河南开封475003 [2]郑州大学基础医学院病理生理教研室,河南郑州450052

出　　处：《中国肿瘤临床与康复》2007年第4期294-296,共3页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：国家自然科学基金资助项目(39870287)

摘　　要：目的研究胃癌组织中DNA聚合酶β基因(polβ)的突变情况。方法利用RT-PCR、SSCP和序列分析对32例胃癌及癌旁组织标本中polβ基因进行检测。结果32例胃癌组织标本中有7例SSCP异常,突变率为21.9%;而对应的32例癌旁组织PCR-SSCP结果中未见异常。在低分化胃癌中的突变率高达50.0%(6/12),而在中、高度分化胃癌中的突变率却仅为5.0%(1/20),经统计学分析,差异有显著性(P<0.05)。测序分析了7例SSCP异常中的3例,其突变分别为196位A→G,268位A→G,790位A→T;测序分析了1例PCR-SSCP无异常的癌旁组织标本,其序列无突变。结论胃癌组织存在polβ基因突变;该基因突变可能与胃癌的发生、发展相关。Objective To study DNA polymeraseβ gene （polβ） mutation in human gastric cancer tissues. Methods DNA polβ gene was examined by RT-PCR , SSCP and sequence analysis. Results There were 7 abnormal SSCP in 32 gastric carcinoma samples,and the mutation rate was 21.9% , while nothing abnormal was found in the tissue adjacent to tumor. The mutation rate was 50.0% （6/12） in low differentiated gastric carcinoma;while it was only 5.0% （1/20） in moderately and highy differentiated gastric carcinoma, with statistically significant difference. Sequence analysis showed that in three of the seven abnormal SSCP samples,the mutations were site 196 A→G, site 268 A→G and site 790 A→T. No mutation was found by seuencing a normal PCR-SSCP sample of the tissue adjacent to tumor. Conclusion It is suggested that the polβ gene mutation may be associated with carcinogenesis and development of human gastric cancer.

关 键 词：DNA聚合酶Β 胃肿瘤 基因突变 测序 

分 类 号：R735.2[医药卫生—肿瘤] R730.45[医药卫生—临床医学]