心搏骤停后脑复苏研究的动物模型  

Establishment of a simple and convenient animal model of cardiac arrest for studying cerebral resuscitaion

作　　者：马宇洁[1] 杨兴易[1] 林兆奋[1] 张雷[1] 潘曙明[2] 

机构地区：[1]第二军医大学附属长征医院急救科,中国人民解放军急救医学中心,上二海200003 [2]上海第二医科大学附属新华医院急救中心

出　　处：《中华急诊医学杂志》2007年第9期957-960,共4页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金(30500173)

摘　　要：目的建立一种新型简便的心跳骤停后脑复苏研究的动物模型。方法选用清洁级雄性Sprague Dawley(SD)大鼠40只,采用窒息合并冰氯化钾停跳液致大鼠心跳骤停,5 min后开始心肺复苏。记录基本生命体征变化、发生循环停止时间(T_(CA))、自主循环恢复时间(T_(ROSC))、72 h存活率,复苏后不同时间点(3、6、12、24、48、72 h)神经功能评分(NDS),观察复苏后3 h和72 h透射电镜下皮层超微结构改变。结果造模后大鼠迅速发生循环停止,T_(CA)和T_(ROSC)分别为(137.3±10.2)s,(64.4±9.3)s,复苏成功率87.5%,72 h存活率为80%。NDS在复苏后3 h时最低,以后NDS渐趋好转。血浆丙二醛(MDA)水平显著升高,在72 h略有回降,但仍高于造模前(P<0.01)。电镜显示复苏后3 h脑损害最严重,72 h逐渐减轻。结论这一模型操作简单且结果稳定。Objective To establish an simple and convenient animal model of cardiac angst for studying cerebral resuscitation. Method Clean male Sprague Dawley rats were randomly divided into sham group and experimental group. Cardiac angst was induced by asphyxiation and ice-cold 0.5 mol KCl with blood flow cut off for 5 minutes. Animals were resuscitated with external cardiopulmonary resuscitation （CPR）, mechanical ventilation, and epinephrine injection. Blood pressure, heart rate, successful ratio of resuscitation after 72 hours, time of cardiac angst （TCA） and return of spontaneous circulation （ TROSC ） were recorded. Neural deficit scores （NDS） and levels of maleic dialdehyde （MDA） in plasma were evaluated at 3, 6, 12, 24, 48, 72 hours after ROSC. The damage score of cortex was measured by transmission electron microscope examinination at 3 hours and 72 hours after ROSC. Results All the rats in experimental group had cardiac arrest rapidly. TCA and TROSC were （137.3±10.2） seconds and （64.4 ± 9.3） seconds, respectively, while the successful rate of resuscitation was 82.5 %. The lowest NDS was at 3 hours after ROSC, while the NDS increased gradually. After CPR, the level of MDA in plasma increased significantly, slightly declined at 72 hours after ROSC, but still significantly higher than before the model. Electron microscope examination of cortex showed neuron slightly, organelle and astrocyte, but became better after 72 hours pest ROSC. Conclusions The model of cardiac angst was easy to establish, and the data provided was accurate, which is useful to study the mechanism of cerebral resuscitation.

关 键 词：心跳骤停 心肺复苏 脑复苏 动物模型 大鼠 

分 类 号：R-332[医药卫生] R459.7