枯草芽孢杆菌甲壳素脱乙酰酶的酶学性质  

Study on the Characteristics of A Chitin Deacetylase

作　　者：黄惠莉[1] 蔡阿娜[1] 陈培钦[1] 

机构地区：[1]工业生物技术福建省高等学校重点实验室(华侨大学),福建泉州362021

出　　处：《氨基酸和生物资源》2007年第3期30-32,46,共4页Amino Acids & Biotic Resources

基　　金：华侨大学校基金资助项目

摘　　要：研究了甲壳素脱乙酰酶的热稳定性及酶的反应体系作用条件:酶(干重)添加量为40 mg.L-1,甲壳素底物(干重)质量浓度为75 mg.L-1,反应时间为90 m in,金属离子Mg2+对酶活有激活作用,在最适宜反应条件下的酶活为2250 U.L-1。甲壳素脱乙酰酶的酶解方式为外切酶型,酶降解终产物对酶活力有抑制作用,酶对甲壳素有一定的降解作用。The characteristics of a chitin deacetylase were studied. The consistency of the enzyme was 40mg · L^-1 , and the consistency of chitin was 75mg · ^-1L. Mg^2＋ enhanced the enzyme activity. The reaction time was 90 minutes. Under the appropriate conditions, the enzyme activity was up to 2250U · mL^-1. The chitin deacetylase showed out -slice, the catabolin by chitin deacetylase had restrainable effect to the enzyme activity.

关 键 词：甲壳素脱乙酰酶 脱乙酰度 酶活 

分 类 号：Q55[生物学—生物化学]