莱克多巴胺抗体的制备与评价  

Production and Evaluation of a Antibody Against Ractopamine

作　　者：王凤侠[1] 张艳[1] 何金兴[1] 王玮[1] 徐蓓[1] 王忠斌[1] 王硕[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津市食品营养与安全重点实验室,天津300457

出　　处：《现代食品科技》2007年第9期1-4,共4页Modern Food Science and Technology

基　　金：国家十一五科技支撑计划(No.2006BAD05A06)

摘　　要：本文以RAC-linker-BSA为免疫抗原获得了莱克多巴胺的多克隆抗体,用混合酸酐法合成包被抗原,建立了莱克多巴胺的间接竞争酶联免疫检测方法(ELISA),并对抗体性质进行了评价。结果表明,制备出的莱克多巴胺抗体具有较高的灵敏度,IC_(50)为0.9ng/mL,检出限(IC_(15))为0.1 ng/mL,低于同类文献的报道结果;除了与多巴酚丁胺的交叉反应为7.5%外,与克伦特罗、沙丁胺醇、特步他林、异丙肾上腺素和异奎胍均无交叉现象,说明抗体特异性较高;此外,抗体具有很好的稳定性,可在4℃冰箱中储存半年以上。为建立酶联免疫快速检测方法,实现对莱克多巴胺的有效监控奠定了良好的基础。An indirect competitive enzyme-linked immunosorbent assay （ELISA） procedure was established to detect ractopamine employing a polyclonal antibody generated from RAC-linker-BSA and a coating antigen synthesized by method of mixed acid anhydride.The antibody against ractopamine showed high sensitivity with an IC50 of 0.9ng/mL and the detection limit （IC15） was 0.1 ng/mL,which was lower than the data reported in literatures.The antibody with high specificity showed no cross-reactivity with clenbuterol,saibutamol,isoproterenol, terbutaline and isoxsuprine,while had the cross-reactivity of 7.5% with dobutamine.In addition,it was stable enough to be stored at 4℃for halfa year.This study laid a well foundation for the control of ractopamine by ELISA method.

关 键 词：莱克多巴胺 ELISA 抗体 

分 类 号：S859.84[农业科学—临床兽医学]