MF/SS受累淋巴结病变的基因诊断  

MOLECULAR DIAGNOSIS IN LYMPH NODES FROM THE PATIENTS WITH MF/SS

作　　者：张春雷[1] 宫恩聪[2] 刘翠玲[2] 沈力[1] 李世荫[1] 

机构地区：[1]北京医科大学第三医院皮肤科 [2]北京医科大学病理学系,100083

出　　处：《北京医科大学学报》1997年第1期48-50,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：探讨基因诊断在确定MF／SS受累淋巴结病变性质中的价值。方法：采用PCR-PAGE技术，研究了20例MF／SS受累淋巴结病变和10例良性皮病性淋巴结病变T淋巴细胞受体基因重排的特点。结果：检测出有TCR-β和／或TCR－γ基因克隆重排的：11／12例（91．7％）典型MF／SS淋巴结病变，5／8例（62．5％）临床病理可疑MF／SS或反应性淋巴结病变。10例良性皮病性淋巴结病变未检测出有TCR个或TCR－γ基因克隆重排，在8例临床病理可疑MF／SS或反应性淋巴结病变病人中，有TCR基因克隆重排的比没有重排的有明显短的生存期。结论：PCR－PAGE检测MF／SS受累淋巴结病变中T淋巴细胞受体基因重排，在其早期诊断及精确分期方面是相当有用的。To study the clinical value of molecular diagnosis in lymph nodes of the patientswith MF/SS. Methods:The polymerase chain reaction(PCR) technique was used to detect the clonal rearrangements of T-cell receptor (TCR) genes in 20 lymph nodes from patients with MF/SS and 10 dermatopathic lymph nodes from patients with benign skin disorders. Results:The dominant clonal TCR-βor γ gene rearrangements were detected in 11/12 (91. 7 % ) MF/SS lymph nodes, 5/8 (62. 5 % ) suspectedor uninvolved dermatopathic MF/SS lymph nodes. No dominant clonal TCR-β or γ gene rearrangementswere detected in 10 benign reactive dermatopathic control lymph nodes. In MF/SS patients with suspected or dermatopathic lymphadenopathy, patients with detectable clone T-cell populations had a significantly shorter survival than patients without such a population. Conclusion:This study demostratedthat the method is extremely useful for the early diagnosis and accurate stage in lymphadenopathy ofMF/SS.

关 键 词：淋巴结疾病 淋巴瘤 基因诊断 

分 类 号：R733.404[医药卫生—肿瘤] R551.204[医药卫生—临床医学]