广西水牛γ干扰素基因的克隆与序列分析  

Cloning and Sequence Analysis of IFN-γ Gene Gene in Guangxi Water Buffalo

作　　者：熊毅[1] 朱伟[2] 徐贤坤[1] 龙剑明[2] 刘棋[1] 

机构地区：[1]广西动物疫病预防控制中心,广西南宁530001 [2]广西大学动物科学技术学院,广西南宁530005

出　　处：《动物医学进展》2007年第9期19-23,共5页Progress In Veterinary Medicine

基　　金：广西科技厅科技攻关项目(0719004-3F)

摘　　要：提取经刀豆素(ConA)诱导培养的水牛外周血淋巴细胞的总RNA,采用RT-PCR技术扩增水牛IFN-γ基因,将其克隆到pMD18-T载体上,并进行测序。序列分析结果表明,获得的IFN-γ基因全长为544个碱基,其开放阅读框(ORF)为501个碱基,编码166个氨基酸。与GenBank上已发表的水牛、乳牛的IFN-γ基因进行同源性比较,其核苷酸同源性均高于97%,氨基酸同源性均高于96%;与其他种类动物的IFN-γ的同源性相比,随其亲缘关系的远近有所降低。Total RNA was isolated from cultured water buffalo peripheral blood lymphocytes stimulated with ConA. The cDNA of water buffalo interferon-γ（IFN-γ） cDNA was amplified by RT-PCR, and then cloned into pMD18-T and sequenced. The ORF of the cloned water buffalo IFN-y gene was 501bp in length, encoding a mature polypeptide with 166 amino acids. Compared with other IFN-γ, the nucleotide homologies between the water buffalo IFN-γ gene and the cattle IFN-γ genes were higher than 97%, the amino acid homologies were higher than 96 %. The homologies with other animals were step down in accordance with its genetic relationship. The results showed that the cloning plasmid was successfully constructed, and it is the basis for further study and application of Guangxi water buffalo IFN-γ.

关 键 词：Γ干扰素 克隆 序列分析 水牛 

分 类 号：Q785[生物学—分子生物学]