Survivin反义寡核苷酸对肝癌细胞增殖和凋亡的影响  

Effect of Antisurvivin Oligonucleotides on Proliferation and Apoptosis of Human Hepatocellular Carcinoma Cell

作　　者：李晓丽[1] 彭利[2] 张萌[2] 徐卓[2] 王顺祥[2] 唐瑞峰[2] 张凤瑞[2] 

机构地区：[1]唐山市工人医院干部病房二病区 [2]河北医科大学第四医院肝胆外科,石家庄市050011

出　　处：《中国肿瘤临床》2007年第16期917-920,共4页Chinese Journal of Clinical Oncology

基　　金：河北省科研基金资助(编号:05138)

摘　　要：目的:观察Survivin反义寡核苷酸对SMMC-7721细胞增殖、凋亡的影响。方法:设计合成特异性Survivin反义寡核苷酸,转染肝癌SMMC-7721细胞,MTT法测定Survivin ASODN对细胞增殖抑制情况的影响,FCM法检测对细胞周期、凋亡及Survivin蛋白表达的影响。结果:Survivin ASODN可抑制SMMC-7721细胞的生长增殖,并呈浓度和时间依赖性。ASODN转染组可诱导SMMC-7721细胞凋亡(P<0.01),细胞周期阻滞于G2/M期(P<0.05)。ASODN转染组Survivin蛋白表达水平显著降低(P<0.01)。结论:Survivin ASODN能下调SMMC-7721细胞Survivin表达,并可抑制其增殖并诱导凋亡。Objective： To investigate the effect of antisense oligonucleotide（ASODN） targeting survivin on apoptosis and proliferation of the SMMC-7721 hepatocellular carcinoma cell line. Methods： ASODN targeting survivin was designed, constructed and transfected to SMMC-7721 cells by oligofectamine. Inhibitory action of the cell growth was measured using MTT assay. Cell cycle, apoptosis and the expressions of survivin protein were detected by flow cytometry. Results： Treatment of with survivin ASODN suppressed cell proliferation significantly in both the timedependent and the dose-dependent manner. The ASODN groups significantly induced apoptosis （P〈0.01） and increased the proportion of cells in the G2/M phase （P〈0.05）. The expression of survivin protein were significantly decreased compared with that of control group （P〈0.01）. Conclusions： The expressions of survivin in SMMC-7721 cell are downregulated by survivin ASODN. ASODN survivin can inhibit the proliferation of the SMMC-7721 hepatocellular carcinoma cells and induce apoptosis.

关 键 词：生存素 反义寡核苷酸 肝细胞癌 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]