酰基辅酶A胆固醇酰基转移酶1基因过表达对泡沫细胞形成的影响  

The Effect of Over-Expressing of Acyl Coenzyme A Cholesteryl Acyltransferase 1 Gene on the Foam Cell Formation

作　　者：何平[1] 成蓓[1] 王洪星 戚本玲[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院老年病科,湖北省武汉市430022 [2]美国Zeneca制药公司分子和细胞生物学部

出　　处：《中国动脉硬化杂志》2007年第5期325-328,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(30170378)

摘　　要：目的研究在三种不同细胞中过表达酰基辅酶A胆固醇酰基转移酶1基因对泡沫细胞形成的影响。方法构建携带酰基辅酶A胆固醇酰基转移酶1全长cDNA的pCDNA3.1质粒载体并稳定转染体外培养的人THP-1单核细胞、小鼠RAW264.7单核巨噬细胞和人胚肾293上皮细胞,以油红O染色法检测在乙酰化低密度脂蛋白作用下转染前后三种细胞形成泡沫细胞的情况。结果在相同的脂质负荷条件下,转染酰基辅酶A胆固醇酰基转移酶1基因的THP-1单核细胞和RAW264.7巨噬细胞同未转染的细胞相比泡沫细胞的形成数量增加,而人胚肾293上皮细胞无论是否转染酰基辅酶A胆固醇酰基转移酶1基因均不易形成泡沫细胞。结论单核巨噬细胞中过表达酰基辅酶A胆固醇酰基转移酶1基因可促进泡沫细胞的形成。Aim To study on the effect of over-expressing acyl coenzyme A cholesteryl acyltransferase-1 （ACAT-1） gene in the formation of foam cells in three kinds of cells. Methods ACAT-1 gene were transfected into cultured human THP-1 monocytic cells, human embryonic kidney 293 （HEK293） cells and murine macrophages RAW264.7 cells. The over-expressing ACAT-1 gene cells were incubated with acetyled low density lipoprotein （ac-LDL） and stained with oil red O to detect the formation of foam cells. Results The formation of foam cells became easier from over-expressing ACAT-1 gene THP-1 monocytic cells and RAW264.7 cells than control. But no apparent changes were observed in over-expressing ACAT-1 gene HEK293 cells. Conclusion Over-expressing ACAT-1 gene accelarated the formation of foam cells in monocytic and macrophagic cell lines.

关 键 词：病理学与病理生理学 酰基辅酶A胆固醇酰基转移酶1 巨噬细胞 单核细胞 泡沫细胞 

分 类 号：R363[医药卫生—病理学]