基质细胞衍生因子1α对氧化型低密度脂蛋白诱导内皮细胞与单核细胞粘附的影响  

Effect of Stromal Cell-Derived Factor-1α on Monocytes and Endothelium Cells Adhesion Induced by Oxidized Low Density Lipoprotein

作　　者：李国华[1] 王佐[1] 王北冰[2] 姜志胜[1] 吕运成[3] 尹凯[2] 危当恒[1] 涂玉林[1] 

机构地区：[1]南华大学心血管病研究所,湖南省衡阳市421001 [2]南华大学诊断学教研室,湖南省衡阳市421001 [3]南华大学解剖学教研室,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2007年第7期487-490,共4页Chinese Journal of Arteriosclerosis

基　　金：第38批中国博士后基金(2005038472)资助;湖南省教育厅基金(04C-581)资助;湖南省自然科学基金(06JJ50051)资助

摘　　要：目的观察基质细胞衍生因子1α对氧化型低密度脂蛋白诱导的单核细胞与内皮细胞粘附的影响。方法逆转录聚合酶链反应法和免疫印迹法分别检测内皮细胞基质细胞衍生因子1αmRNA和蛋白的表达,静态粘附实验观察氧化型低密度脂蛋白和基质细胞衍生因子1α抗体干预对内皮细胞与单核细胞粘附的影响。结果基质细胞衍生因子1α在静息状态下的血管内皮细胞上几乎不表达,随着氧化型低密度脂蛋白浓度增加和处理时间延长,基质细胞衍生因子1α的mRNA和蛋白表达水平均明显上调,25mg/L氧化型低密度脂蛋白处理48h时,基质细胞衍生因子1α表达到达峰值。氧化型低密度脂蛋白浓度为1、5、25及125mg/L时,每个视野粘附的单核细胞数分别为190±15、226±23、280±14、253±8,而正常对照组为104±10,差异有显著性意义(P<0.05)。终浓度为0.01、0.1和1μg/L基质细胞衍生因子1α抗体干预后,粘附的单核细胞数分别为202±17、142±6和115±12,明显低于对照组的279±11(P<0.05)。结论基质细胞衍生因子1α参与了内皮细胞与单核细胞的粘附。Aim To investigate the adhesion regulation effect of stromal cell-derived factor 1α （SDF-1α） on monocytes and endothelial cells induced by oxidized low density lipoprotein （ox-LDL）. Methods SDF-1α mRNA and protein were measured by reverse transcript-polymerase chain reaction （RT-PCR） and Western blot respectively in endothelial cells. Cell counting was used to observe the effect of the monocytes and endothelial cells adhesion treated with ox-LDL or SDF-1α antibody. Results SDF-1α was hardly expressed in normal endothelium, but was up-regulated by ox-LDL in a dose and time dependent manner, It peaked at 25 mg/L ox-LDL for 48 h. The adhered amount of monocytes to endothelial cells were 190±15, 226±23, 280±14, 253±8 respectively responding to 1, 5 and 125 mg/L ox-LDL. And the amount was 104±10 in the control （P〈0.05）. While endothelial cells pretreated with ox-LDL were incubated with SDF-1α antibody with 0.01, 0.1, 1 μg/L respectively, the adhesion of monocytes was 202±17, 142±6, 115±12. It was lower than that of control group significantly （279±11; P〈0.05）. Conclusion SDF-1α is implicated in monocytes and endothelium cells adhesion.

关 键 词：病理学与病理生理学 内皮细胞 单核细胞 氧化型低密度脂蛋白 基质细胞衍生因子1Α 

分 类 号：R363[医药卫生—病理学]