三株白腐菌产锰过氧化物酶活性及其对多环芳烃的降解  

Effect of cyclodextrin on PAHs degradation by MnP enzymes produced by Phanerochaete chrysosporium

作　　者：丁娟[1] 罗坤[1] 周娟[1] 高士祥[1] 

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,江苏南京210093

出　　处：《环境污染与防治》2007年第9期656-660,共5页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.20677024)

摘　　要：比较研究了β-环糊精（β-CD）对3株黄孢原毛平革菌（Phanerochaete chrysosporium）CGMCC5.776、GIM3.383和GIM3.393产锰过氧化物酶（MnP）和降解多环芳烃（PAHs）的影响。结果表明：3株菌都能分泌出高活性的MnP,β-CD能显著提高MnP的活性,加入2.0 g/Lβ-CD的体系培养到第12天时,酶活性最高达到2 912.5 U/L;而且β-CD能显著提高PAHs的降解率,两者之间存在较好的相关性,加入1.0 g/Lβ-CD的体系对比不加β-CD的体系,PAHs的降解率提高了1～2倍。PAHs的降解同时受到MnP活性、PAHs表观溶解度和分子结构稳定性等因素的影响。Three isolates of Phanerochaete chrysosporium were capable of producing manganese peroxidase （MnP） which was effective for degrading polycyclic aromatic hydrocarbons （PAHs）. The effects of β-cyclodextrin （β-CD） on the isolates＇ production of MnP and the resulting PAHs degradation were determined. The presence of β-CD enhanced both the MnP production and the PAHs degradation; increasing the β-CD dose made the PAHs more water soluble and the enzyme more active resulting in a higher PAHs degradation rate. The highest MnP activity （2 912.5 U/L） was observed after 12-day in the test system with 2.0 g/L of β-CD, and the PAHs degradation rate increased by 1 ～2 folds after adding 1.0 g/L β-CD.

关 键 词：黄孢原毛平革菌 锰过氧化物酶 多环芳烃 Β-环糊精 

分 类 号：X172[环境科学与工程—环境科学]