转基因水稻潮酶素标记基因在大鼠体内代谢的初步研究  

Primary investigation on metabolism of hptgene in transgenic rice modified with cowpea trypsin inhibitor gene in rats

作　　者：徐海滨[1] 殷昭雪[2] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021 [2]首都医科大学

出　　处：《卫生研究》2007年第5期559-563,共5页Journal of Hygiene Research

基　　金：国家科技部973计划(No.2001CB109007)

摘　　要：目的初步探索hpt在大鼠消化道内降解的特点和肝脏、肾脏转移的可能性。方法48只成年雄性SD大鼠随机分为8组,每组6只,6组作为转基因组,2组作为非转基因组,分别喂饲转基因饲料和非转基因饲料两周。所有动物于试验结束当天喂饲后2、4、6、8、10和24h处死动物,取胃、空肠、回肠下段、盲肠、直肠的内容物。另取20只雄性SD大鼠随机分为2组,每组10只,转基因大米饲料与非转基因饲料喂饲大鼠4周,取其肝脏、肾脏。用PCR方法检测hpt片段在肠道和肝、肾脏器中的代谢情况。结果hpt基因在胃内容物中有少量未被降解之外,在小肠、盲肠及直肠内容物中,基因大部分已被降解。hpt基因在肠道的降解存在时间和片断依赖型。在试验周期内,肝、肾脏器中未捡出hpt基因片断。结论转基因水稻hpt基因在大鼠消化道内很容易降解,且不能在肝、肾脏器中被捡出。Objective The metabolism characteristic in the gastrointestinal tract and possibility of transfer from gastrointestinal tract into liver and kidney was investigated. Methods 48 SD rats were allotted into 8 subgroups randomly, 6 groups rats of them were fed with transgenic rice diets and 2 groups rats were fed with non-transgenic rice diets. All rats were fed with transgenic and non-transgenic rice for two weeks. All rats were fast and sacrificed, the contents of stomach, jejunum, the lower part of ileum, cecum and rectum were taken out at 2, 4, 6, 8, 10 and 24 hours respectively after eating final diets. Fed two groups of SD rats with transgenic diet and non-transgenic rice diet respectively for 4 weeks, the liver and kidney were sampled and stored. All samples was examined by PCR. Results The fragment of hpt was found the was only detected in stomach and jejunum, but none of the five fragments was detected in the lower part of ileum, cecum and rectum. The degradation of hpt was time-relationship and long-length fragment relationship, the longer was the action time, the more serious was the degradation. Foreign hpt gene was not detected in liver and kidney. Conclusion hpt gene was easy to degraded in gastrointestinal trace and was not detectable in hepatic tissue and nephridial tissues.

关 键 词：转基因 水稻 标记基因 代谢 安全性评价 

分 类 号：R155.51[医药卫生—营养与食品卫生学] TS201.6[医药卫生—公共卫生与预防医学]