发酵法制备1,6-二磷酸果糖Ⅰ发酵菌株的确定及反应条件的研究  

PREPARATION OF FRUCTOSE-1.6-DIPHOSPHATE BY FERMENTATION I DETERMINATION OF STRAINS AND STUDIES OF INCUBATION CONDITIONS

作　　者：阎浩林[1] 苏昕[1] 王骅丽 

机构地区：[1]沈阳药科大学制药系,110015

出　　处：《微生物学杂志》1997年第1期10-13,共4页Journal of Microbiology

摘　　要：通过比较6株酵母细胞转化1，6－二磷酸果糖的结果，确定酿造酵母Y162作为转化菌株，对其菌株的培养方法，底物反应额的组成及反应条件进行了详细的考察，在最适条件下：pH6.5，蔗糖8％，NaH2PO45％，MgCl2·6H2O0.1％，甲苯4.0％，氯丙嗪0.2％，反应时间为8-10h，湿菌体与反应液的比例为1：4，反应液中FDP的产量可达12．1mg／ml。Saccharomyces cerevisiae Y 162 was finally used as conversion strain after comparing the ability of 6 Jreast strains to transform from sucrose to FDP. The cultural methods, components of culture fluid and incubation conditions were all car 3 fully studied.The inost optimum conditions for producing FDP were as follow: pH 6. 5, sucrose 8％, NaH2PO4 5％, MgCl26H2O 0. 1％, toluene 4.0％, chlorderazin 0.2％, incubation period was 8-10 hours. When the ratio of wet cells to culture fluid was 1: 4, the maximum FDP accttmulation in the incubation fluid could be attained a9 much as 12. 1mg/ml.

关 键 词：二磷酸果糖 发酵 制备 

分 类 号：TQ929[轻工技术与工程—发酵工程]