Effects of vegetation type on soil microbial community structure and catabolic diversity assessed by polyphasic methods in North China  

作　　者：HAN Xue-mei WANG Ren-qing LIU Jian WANG Meng-cheng ZHOU Juan GUO Wei-hua 

机构地区：[1]School of Life Sciences, Shandong University, Jinan 250100, China [2]Institute of Environment Research, Shandong University, Jinan 250100, China

出　　处：《Journal of Environmental Sciences》2007年第10期1228-1234,共7页环境科学学报（英文版）

基　　金：Project supported by the Outstanding Young Scientists Foundation Grant of Shandong Province (No.2005BS08010);China Geological Survey Project (No.1212010310306);Key Project of Natural Science Foundation of Shandong Province (No.Z2006D04).

摘　　要：Soil microbes play a major role in ecological processes and are closely associated with the aboveground plant community. In order to understand the effects of vegetation type on the characteristics of soil microbial communities, the soil microbial communities were assessed by plate counts, phospholipid fatty acid （PLFA） and Biolog microplate techniques in five plant communities, i.e., soybean field （SF）, artificial turf （AT）, artificial shrub （AS）, natural shrub （NS）, and maize field （MF） in Jinan, Shandong Province, North China. The results showed that plant diversity had little discernible effect on microbial biomass but a positive impact on the evenness of utilized substrates in Biolog microplate. Legumes could significantly enhance the number of cultural microorganisms, microbial biomass, and community catabolic diversity. Except for SF dominated by legumes, the biomass of fungi and the catabolic diversity of microbial community were higher in less disturbed soil beneath NS than in frequently disturbed soils beneath the other vegetation types. These results confirmed that high number of plant species, legumes, and natural vegetation types tend to support soil microbial communities with higher function. The present study also found a significant correlation between the number of cultured bacteria and catabolic diversity of the bacterial community. Different research methods led to varied results in this study. The combination of several approaches is recommended for accurately describing the characteristics of microbial communities in many respects.Soil microbes play a major role in ecological processes and are closely associated with the aboveground plant community. In order to understand the effects of vegetation type on the characteristics of soil microbial communities, the soil microbial communities were assessed by plate counts, phospholipid fatty acid （PLFA） and Biolog microplate techniques in five plant communities, i.e., soybean field （SF）, artificial turf （AT）, artificial shrub （AS）, natural shrub （NS）, and maize field （MF） in Jinan, Shandong Province, North China. The results showed that plant diversity had little discernible effect on microbial biomass but a positive impact on the evenness of utilized substrates in Biolog microplate. Legumes could significantly enhance the number of cultural microorganisms, microbial biomass, and community catabolic diversity. Except for SF dominated by legumes, the biomass of fungi and the catabolic diversity of microbial community were higher in less disturbed soil beneath NS than in frequently disturbed soils beneath the other vegetation types. These results confirmed that high number of plant species, legumes, and natural vegetation types tend to support soil microbial communities with higher function. The present study also found a significant correlation between the number of cultured bacteria and catabolic diversity of the bacterial community. Different research methods led to varied results in this study. The combination of several approaches is recommended for accurately describing the characteristics of microbial communities in many respects.

关 键 词：BIOLOG LEGUME microbial biomass plant diversity phospholipid fatty acid （PLFA） soil microbes 

分 类 号：S154.3[农业科学—土壤学]