莴苣叶多酚类物质的提取及其清除超氧阴离子与羟基自由基能力评价  

Extraction of polyphenol from lettuce leaves and evaluation of the scavenging ability of polyphenol on superoxide anion and hydroxyl radical

作　　者：曾凡梅[1] 殷红军[1] 

机构地区：[1]长江大学生命科学学院,湖北荆州434025

出　　处：《江西食品工业》2007年第3期33-36,共4页Jiangxi Food Industry

摘　　要：本文研究莴苣叶多酚的提取工艺,评价其清除超氧阴离子(O2-·)与羟基自由基(·OH)的能力,探讨开发利用莴苣叶的途径。多酚含量用Folin-酚法测定,通过单因素试验及正交试验优化乙醇溶液提取莴苣叶多酚的工艺条件。分别用邻苯三酚自氧化法与Fenton反应测定莴苣叶多酚清除O2-·与·OH的能力。结果表明:以70%乙醇为提取溶液、料液比1∶9、在50℃下提取30min,莴苣叶多酚的提取率最高,达680.5mg/100g。在0.5mg/mL～2.5mg/mL范围内,莴苣叶多酚对O2-·清除率随浓度上升而显著提高,2.5mg/mL的莴苣叶多酚对O2-·的清除率已超过90%。在0.5mg/mL～2.0mg/mL范围内,莴苣叶多酚对·OH清除率随浓度上升而显著提高,2.0mg/mL的莴苣叶多酚对·OH清除率已超过90%。In this paper the processing of extracting polyphenol from lettuce leaves was studied and the scavenging abilities of polypehnol on superoxide anion and hydroxyl radical were evaluated to develop method of utilizing lettuce leaves. The polyphenol content was measured by Folin-hydroxybenzene assay , and the extracting processing was optimized by the single factor and orthogonal test. The scavenging abilities on superoxide anion and hydroxyl radical were measured by self-oxidationof1,2,3-phentriolassay and Fenton reactions respectively. Results showed that 70% ethanol solution and material to solution being 1：9 and 50℃plus 30 minutes are the optimal conditions for extraction with the highest extracting rate of 680.Smg/lOOg. Between 0.5 mg/mL and 2.5 mg/mL , the scavenging rate on superoxide anion radical raised significantly with the concentration increased, and the scavenging rate of 2.5 mg/mL polyphenol was higher than 90%. There have similar phenomena to polyphenol scavenging hydroxyl radical, but the concentration changed to 0.5 mg/mL to 2.5 mg/mL.

关 键 词：莴苣叶 多酚 提取 超氧阴离子自由基(O^-2·) 羟基自由基(·OH) 

分 类 号：TS202.3[轻工技术与工程—食品科学]