胃癌中hMLH1基因突变及其与HP感染的关系  

hMLH1 mutation and the correlation with HP infection in gastric carcinoma

作　　者：王丽珍[1] 李春鸣[2] 刘连[2] 肖庆帮[2] 

机构地区：[1]皖南医学院病理学教研室,芜湖241001 [2]遵义医学院附属医院病理科,563003

出　　处：《贵州医药》2007年第9期774-776,共3页Guizhou Medical Journal

基　　金：贵州省优秀青年人才培养计划项目[黔科合人字(2005)0512]

摘　　要：目的探讨hMLHl基因突变与胃癌临床病理特征的关系及HP致胃癌的可能机制。方法应用激光捕获显微切割技术获取所需细胞,聚合酶链反应-单链构象多态性分析(PCR-SS-CP)银染技术检测49例胃癌hMLH1基因突变,用Warthin-Starry银染法观察胃癌组织HP感染情况。结果胃癌组织中hMLH1基因5个外显子总突变率为6.12%,hMLH1突变率与胃癌患者的年龄、性别、发生部位、有无淋巴结转移、组织学类型、分化程度及病理变化进展程度均无显著关联(P>0.05)。HP感染胃癌组与非感染胃癌组hMLH1突变率差异无显著性(P>0.05)。结论hMLH1基因突变可能参与了部分胃癌的发生,其与胃癌的临床病理特征无显著关联;本实验尚未发现HP感染与hMLH1基因突变间的显著关联。To study the hMLH1 mutation , clinicopathological characteristics and the possible mechanism of carcinogenesis caused by HP infection in gastric carcinoma. Methods The hMLH1 mutation was measured by PCR-SSCP and silver-staining. HP infection was examined by Warthin-Starry method. Results The hMLH1 mutation rate was 6.12% in gastric carcinoma and it was not significantly correlated to gastric clinicopathological characteristics. There was no significant difference between HP infection and without HP infection. Conclusion The hMLH1 gene mutation may be involved in the pathogenesis of gastric carcinoma. There were no significant correlation of hMLH1 mutation and clinicopathological characteristics of gastric carcinomas.

关 键 词：胃肿瘤hMLHl 突变 幽门螺杆菌 

分 类 号：R735.2[医药卫生—肿瘤]