刺五加对巨噬细胞产生NO与肿瘤坏死因子-α的抑制作用  

Inhibitory Effect of Acanthopanax senticosus on the Production of Nitric Oxide and Tumor Necrosis Factor-α in RAW 264.7 Macrophages

作　　者：林秋叶[1] 曹振辉[2] 姜春生 张久石 徐永平[1] 

机构地区：[1]大连理工大学生物科学与工程系,辽宁大连116023 [2]云南农业大学动物科学学院,云南昆明650201 [3]大连赛姆生物工程公司,辽宁大连116620

出　　处：《安徽农业科学》2007年第26期8088-8089,8113,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(06C262222120113);国家杰出青年基金资助项目(30125034);国家高技术研究发展计划(2006AA10Z412);国家科技型中小企业技术创新基金(06C26222120113)。

摘　　要：用不同浓度刺五加提取物培养RAW264.7巨噬细胞,采用LPS与IFN-γ作为诱导剂,37℃培养24h,Griess试剂检测培养上清液中的NO含量,ELISA法检测培养上清液中的TNF-α含量。结果表明:刺五加提取物在浓度10～1000mg/L内对RAW264.7巨噬细胞分泌的NO具有极显著抑制作用(P<0.01),且呈剂量和时间依赖关系;刺五加提取物对TNF-α的分泌没有影响。这表明刺五加提取物可以显著抑制RAW264.7巨噬细胞产生的NO,而对肿瘤坏死因子-α的产生无明显抑制作用。[Objective] The paper was conducted to investigate the effect of Acanthopanax senticosus extracts on the production of nitric oxide （NO） and tumor necrosis factor-α （TNF-α） in RAW264.7 macrophages induced by lipopolysaccharides （LPS） and interferon-gamma （IFN-γ）. [Methods] RAW264.7 macrophages were incubated with LPS and IFN-γ in the presence or absence of Acanthopanax senticosus extracts at various concentrations （1,10,100, or 1000 mg/L ） for 24 h at 37 ℃. The contents of nitrite and TNF-α in the medium were determined by Griess Reagent and ELISA, respectively. [Results] Acanthopanax senticosus extracts （10, 100, or 1000 mg/ L） dose-dependently significantly inhibited NO production in RAW264.7 macrophages induced by LPS and IFN-γ. However, exposure of RAW264.7 macrophages to Acanthopanax senticosus extracts did not affect TNF-α production. [Conclusion] Exposure of RAW264.7 macrophages to Acanthopanax senticosus extracts inhibited NO production induced by LPS and IFN-γ, but had no significant inhibitory effect on the production of tumor necrosis factor-α.

关 键 词：刺五加 RAW264.7巨噬细胞 一氧化氮 肿瘤坏死因子-Α 

分 类 号：Q949.9[生物学—植物学]