保健食品中3种蒽醌衍生物含量的高效液相色谱法测定  

Determination of three kinds of anthraquinone derivatives in health foods by HPLC

作　　者：郭健[1] 李敏[1] 张晓炜[1] 

机构地区：[1]辽宁省疾病预防控制中心,沈阳110005

出　　处：《中国卫生检验杂志》2007年第9期1596-1597,1620,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立RP—HPLC法测定保健食品中3种蒽醌衍生物含量。方法：以XTerra^TM RP18（3．9mm×150mm，5μm）为色谱柱，流动相：甲醇＋含0．5％冰醋酸水溶液（75＋25），检测波长：270nm，流速：1ml／min，柱温：25℃，外标法定量。结果：同时测定3种蒽醌衍生物大黄酸、大黄酚、大黄素的含量，其浓度分别在5．4～54、7．5-75和8．8～88μg／ml范围内呈良好的线性关系。方法的平均回收率分别为：107．3％、99．7％、101．1％，RSD为4．2％、6．8％、7．3％。结论：该方法简便、快速，测定结果准确可靠，重现性好，可用于保健食品中蒽醌含量的测定。Objective：To establish a RP- HPLC method for simultaneous determination of three kinds of anthraquinone derivatives in Health foods. Methods：Anthraquinone derivatives were determined by HPLC using XTerraTM RPls （3.9 mm×150 mm, 5 μm） ,0. 5% acetic acid - methanol （25 -75） ,detective wave 270 nm,flow rate 1 ml/min and column temperature 25℃. Results： The linearity of Rhein, Chrysophanol, Emodin were good in the range of 5. 4 - 54,7.5 - 75 and 8.8 - 88 μg/ml. The average recovery of Rhein was 107.3%, Chrysophanol 99.7%, Emodin 101.1%. Relative standard deviations （RSD） of them were 4. 2% ,6. 8% ,7.3%. Condusion： This method is simple, rapid, accurate and reproducible for detection of anthraquinone in Health foods.

关 键 词：高效液相色谱法 保健食品 蒽醌 

分 类 号：O657.72[理学—分析化学]