Rac1活化在大肠癌细胞SW480迁移侵袭中的作用  

Role of Racl activation in migration and invasion of colorectai cancer cell line SW480

作　　者：南清振[1] 高蕾 张振书[1] 

机构地区：[1]南方医科大学南方医院消化系疾病研究所,广州510515 [2]广州中医药大学第二附属医院影像科

出　　处：《中华肿瘤杂志》2007年第9期666-669,共4页Chinese Journal of Oncology

摘　　要：目的研究Racl的活化在大肠癌迁移侵袭中的作用。方法对大肠癌SW480细胞株,分别导入活化的Rac1 L61重组体和对照用质粒;采用配体结合免疫共沉淀法通过Western blot检测细胞中活化Rac1的含量,进一步用。Transwell小室研究具有不同活性Rac1的SW480细胞其迁移及侵袭能力。结果SW480细胞的转染效率高达80%以上,配体结合免疫共沉淀结果显示,转染Rac1 L61重组体后Rac1的活性显著高于对照组;应用Transwell小室对SW480细胞迁移及侵袭能力研究时,发现Rac1活性高的实验组迁移及侵袭的细胞数显著高于对照组(迁移细胞数43±9:22±5, P<0.01;侵袭细胞数73±13:38±1,P<0.01)。结论Rac1的活化在大肠癌迁移侵袭中起重要的作用。Objective To study the role of activation of Racl in colorectal cancer cell migration and invasion. Methods Racl U61 plasmid and control plasmid were transfected into colorectal cancer cell line SW480 cells. Pull down assay by Western blotting was carried out to measure the amount of activited Racl, and transwell permeable supports were used to assess the migration and invasion of SW480 cells with different activitivity of Racl. Results The transfection ratio of SW480 cells was more than 80%. Pull down assay showed that the activited Racl was significantly higher in the SW480 cells transfected with Racl 161 plasmid than that in the control, and the amount of migrating and invasing SW480 cells transfected with Racl L61 plasmid in the Transwell permeable supports were significantly more than those in controls （ migrating cell numbers：43 ±9vs.22±5,P〈0.01;invasingcellnumbers：73 ±13vs.38 ±1,P〈0.01）. Conclusion The activation of Racl plays an important role in the migration and invasion of colorectal cancer cells.

关 键 词：大肠肿瘤 迁移 侵袭 RACL SW480细胞 

分 类 号：R735.34[医药卫生—肿瘤]