喉癌前病变及喉鳞状细胞癌微卫星杂合性缺失的变化及其意义  

The changes and significance of microsatellite loss of heterozygosity in laryngeal premalignant lesions and squamous cell carcinoma

作　　者：郭涛[1] 孙敬武[1] 

机构地区：[1]安徽医科大学附属省立医院耳鼻咽喉头颈外科,合肥230001

出　　处：《临床耳鼻咽喉头颈外科杂志》2007年第18期831-833,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨喉癌前病变及喉鳞状细胞癌病变组织上杂合性缺失(LOH)的特征及其意义。方法:选取染色体3p、9p和17p上6个多态性微卫星位点D3S1234、D9S171、D9S1748、D9S162、INFA和p53,利用聚合酶链式反应-简单序列长度多态性-银染技术,对49例喉癌前病变和喉癌组织进行LOH分析。结果:6个微卫星标记物LOH发生率分别为:单纯过度增生3.70%,轻度不典型增生10.81%,重度不典型增生26.03%,喉鳞状细胞癌38.67%。其中LOH的总检出率在不同病理组间差异有统计学意义(χ2=17.686,P<0.01),其频率随病变程度加重而明显升高。6个多态性微卫星位点中,LOH发生率最高的位点D9S171(35.00%)。结论:基因水平的改变发生在喉癌变的早期阶段,微卫星标志物可能成为喉癌前病变早期诊断的有用标志物。Objective To evaluate the character and significance of the loss of heterozygosity（LOH） on chromosomes 3P 9P and 17P in laryngeal premalignant and malignant lesions. Method.. Allelic deletions analysis was performed with 6 polymorphic markers（D3S1234, D9S171, D9S1748, D9S162, INFA and p53） using polymerase chain reaction-single sequence length polymorphism-silver staining in 49 laryngeal premalignant and malignant lesions. Result： Allelic loss was seen in 3.70 % of the six markers in patients with simple hyperplasias（HP）, 10.81 with low grade dysplasia（LGD）, 26.03% with high grade dysplasia（HGD） and 38.67% with laryngeal squamous cell carcinoma（LSCC）. Significant differences was found between allelic loss and clinical pathological grades（X^2= 17. 686, P〈0. 01）. The rate of LOH increased remarkably with the lesions progressed from HP to LSCC. D9 S171 showed the highest incidence of LOH （35.00% ） on the six markers. Conclusion： Microsatellite DNA LOHs occur in early stage of laryngeal carcinogenesis. Microsatellite markers may be useful in the early diagnosis for laryngeal premalignant lesions.

关 键 词：喉肿瘤 癌 鳞状细胞 癌前病变 微卫星DNA 杂合性缺失 

分 类 号：R739.65[医药卫生—肿瘤]