根瘤菌N613胞外多糖发酵条件及抗肿瘤作用研究  

Studies on the Fermentation Conditions and Anti-tumor Effect of Exopolysaccharide from Rhizobium sp.N613

作　　者：韩勇[1] 黄晓波[1] 董岳峰[1] 程红兵[1] 赵良启[1] 

机构地区：[1]山西大学化学生物学与分子工程教育部重点实验室,太原030006

出　　处：《微生物学通报》2007年第5期909-913,共5页Microbiology China

基　　金：山西省自然科学基金资助项目(No2006011073);山西高校科技研究开发资助项目(No20051203)

摘　　要：用摇瓶正交试验研究了根瘤菌Rhizobium sp.N613合成根瘤菌胞外多糖(rhizobium exopolysaccharide,REPS)的最佳培养基配方和最适培养条件。采用10L自动控制罐进行了分批发酵试验,获得了合成REPS的发酵动力学参数。经40h的补料分批发酵与代谢调控,REPS产量达到11.31g/L。此外,抗肿瘤实验表明,当REPS剂量为5mg/kg时,其抑瘤率可达53.40%。结果表明,REPS的发酵周期短,产量高,成本低,且具有良好的免疫活性与增稠性,有着极高的开发应用价值。The potential of Rhizobium sp. N613 to produce the exopolysaccharide （REPS） was studied in this paper. Using an orthogonal design in a flask-shaker culture system, the fermentation medium and conditions of synthesizing REPS were optimized. Based on these results, the fermentation kinetic parameters were obtained in the batch fermentation with a 10L fermentor. The REPS yield of 11.31g/L was achieved by metabolic regulation during 40 h fed-batch fermentation. Transplanted tumor models of sarcoma 180 in mice were used to evaluate the anti-tumor effect. The result of antitumor activities showed that inhibition rate was 53.40%, when dose of REPS was 5mg/kg. These results indicate that REPS has the following properties： the short duration of fermentation, the high yield, the low cost, the effective immunoeompetenee and thickening. Thus, REPS has the value of development and application.

关 键 词：根瘤菌 胞外多糖 优化 发酵 抗肿瘤 

分 类 号：R91[医药卫生—药学] R96