E-cadherin基因在卵巢癌高低转移细胞中的差异表达及其机制的探讨  

Different expression of E-cadherin in human ovarian cancer cell lines with highly and lowly metastatic possibilities

作　　者：宋阳[1] 胡军[1] 郝立宏[1] 赵瑾瑶[1] 宫琳琳[1] 邵淑娟[1] 

机构地区：[1]大连医科大学组织胚胎学教研室,辽宁大连116044

出　　处：《大连医科大学学报》2007年第5期425-428,共4页Journal of Dalian Medical University

基　　金：国家自然科学基金(30672392);辽宁省自然科学基金(20062161)

摘　　要：[目的]探讨上皮性细胞黏附分子在人卵巢浆液性囊腺癌高、低转移细胞株HO-8910和HO-8910PM的表达情况。[方法]采用蛋白印迹及免疫细胞化学方法检测2种细胞株的E-cadherin蛋白表达差异,用RT-PCR法检测2种细胞株的mRNA表达差异,用PCR及基因测序法检测基因突变情况。[结果]E-cad蛋白及mRNA在HO-8910中表达,在HO-8910PM中不表达。在E-cadherin基因外显子6~9未发现突变。[结论]E-cad的表达可能与肿瘤的转移能力负相关。[ Objective] To investigate the different expressions of E -cadherin in high and low metastatic human ovary serous cystadenocarcinoma cells. [ Methods] The protein expressions of E- cadherin were detected by western blotting and immunocytochemistry. The mRNA expressions were detected by RT - PCR. The mutations of the gene were detected by PCR and gene sequencing. [ Results] The protein and mRNA of E - cadherin were detected in HO - 8910, but were not detected in HO - 8910PM. The mutations existing in exon 6 -9 of E -cadherin were not detected. [ Conclusion] The expression of E -cadherin may be negatively related to metastasis of the tumor.

关 键 词：E-钙黏附蛋白 基因突变 肿瘤转移 

分 类 号：R737.31[医药卫生—肿瘤]