体积排阻色谱与电感耦合等离子体质谱联用技术在富硒金针菇硒多糖形态分析中的应用研究  

Se-polysaccharides Speciation in Flammulina Velutipes by SE-HPLC-ICP-MS

作　　者：铁梅[1] 李闯[1] 费金岩[1] 李华为[2] 臧树良[1] 

机构地区：[1]辽宁大学环境科学系,沈阳110036 [2]沈阳师范大学化学与生命科学学院,沈阳110034

出　　处：《分析科学学报》2007年第5期497-501,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.20271024)

摘　　要：提取含硒7.97μg/g的富硒金针菇硒多糖,采用体积排阻色谱与电感耦合等离子体质谱(SE-HPLC-ICP-MS)联用技术进行在线分离分析,确定富硒金针菇中至少含有三种硒多糖,其分子量分别为4.27 kDa1、.868 kDa和1.54 kDa,含硒量分别占可溶性硒多糖中硒含量的14.95%、40.39%和44.66%。进一步研究证明:在金针菇的富硒培养过程中,硒不仅参与了生物大分子的合成,而且在富硒金针菇体内存在大量的具有生物活性的硒多糖。从分子生物学角度对新的补硒资源和生物活性因子进行有益的探索,为研究人类硒源的生物安全性和高效可利用性提供科学的理论依据。Se-polysaccharides in which the Se concentration is 7.97 μg/g were extracted in Se-enriched Flammulina Velutipes. SE-HPLC-ICP-MS hyphenated technique was used to separate and investigate the speciation of Se-polysaccharides. Three types of polysaccharides containing Se were found in Seenriched Flammulina Velutipes, and their molecular weight were 4. 27 kDa, 1. 868 kDa and 1.54 kDa, respectively. Se content in above Se-polysaccharides accounted for 14.95% ,40.39% ,44.66% of total Se in soluble Se-polysaccharides. It indicated that Se participated in the synthesis of hiomacro molecules and inorganic Se could be turned into organic Se with bioactivity in the growth of Flammulina Velutipes. Therefore, Flammulina Velutipes is possible to become a new Se source.

关 键 词：硒多糖 金针菇 形态分析 体积排阻色谱-电感耦合等离子体质谱 

分 类 号：O657.63[理学—分析化学]