用DSC测定小鼠看家基因β-actin的PCR反应体系的比热  

Specific Heat Capacity Assay of Mouse β-actingene PCR System Using DSC

作　　者：周国燕[1] 陈唯实[1] 冉姝[1] 林舒律[1] 卜剑鸣[1] 华泽钊[1] 

机构地区：[1]上海理工大学食品与低温生物技术研究所,上海200093

出　　处：《食品科学》2007年第10期348-351,共4页Food Science

基　　金：国家自然科学基金项目(50676063);上海市重点学科建设项目(T0503)

摘　　要：本实验用差示扫描量热技术,测量了不同反应循环数的以PCR内参小鼠看家基因β-actin为目的序列的扩增体系在30～94℃温度范围内的比热容。实验发现,在扩增的初期(3～15个循环),反应体系的比热随着循环数的增加,变化不大;在扩增的指数增长期(>20个循环)比热先降低后增加。同一循环,比热随着温度的增加而增加。未经过PCR扩增的反应体系的玻璃化转变温度为-15.29℃。The specific heat capacity of mouse β-actin gene system in PCR（polymerase chain reaction） was studied in this paper. By the differential scanning calorimeter （DSC）, the specific heat of PCR amplified system with different PCR cycles was assayed. Further, the glass transition temperature of the PCR amplified system was also investigated in the tempera- ture range of -60～50 ℃. Experimental results showed that the more are the PCR cycles, the lower the peak value of the specific heat capacity, but the larger after 26 cycles due to the content of DNA duplexes increasing. The glass transition temperature of PCR system was -15.29 ℃.

关 键 词：小鼠看家基因 差示扫描量热分析(DSC) 比热 玻璃化转变温度 

分 类 号：Q781[生物学—分子生物学]