液相色谱-荧光检测法同时测定肠衣中八种磺胺类兽药残留  

Simultaneous Determination of Eight Kinds of Sulfonamide Residues in Casing by Liquid Chromatography- fluorescence

作　　者：高文惠[1] 王凤池[2] 吕红英[2] 罗敏[1] 胡静[1] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018 [2]河北出入境检验检疫局,河北石家庄050071

出　　处：《食品科学》2007年第10期430-432,共3页Food Science

基　　金：河北省博士基金项目(06547003D-3);河北省教育厅课题(Z2005202);河北科技大学杰出青年基金项目(2006JC-14)

摘　　要：建立了一种专属性强的高效液相色谱—同时测定肠衣中八种磺胺类药物残留(磺胺嘧啶、磺胺间甲氧嘧啶、磺胺甲基嘧啶、磺胺对甲氧嘧啶、磺胺喹恶啉、磺胺甲基异恶唑、磺胺甲氧嗪和磺胺二甲嘧啶)的检测方法。采用1%乙酸/乙腈对肠衣中的磺胺类药物残留进行提取,用正己烷净化,净化后的样品,经荧光胺衍生,供液相色谱仪测定。实验结果表明,八种磺胺药物在2.00～5.00×102ng/ml浓度范围内,具有良好的线性关系,线性相关系数在0.9982～0.9997,添加浓度为5～50ng/ml时,回收率在63.7%～87.8%之间,该方法的最低检测限为5ng/ml,完全可以达到日常检测要求。A method was developed for the determination of eight kinds of sulfonamide residues （sausagessulfadiazine, sulfamonomethoxine,sulfamethoxazole, sulfamerazine, sulfamethoxydiazine, sulfaquinoxaline, sulfamethoxypyridazine and sulfadimidine）in casing by liquid chromatography-fluorescence. The sulfonamide residues in casing were extracted with 1% acetic acid /acetonitrile and cleaned up by n-hexane and derived with fluorescamine for LC analysis. Results showed that the calibration curve had good linear in the ranges of 2.00×10^2～5.00×10^2 ng/ml, and the correlation coefficients were 0.9982～0.9997 for 8 sulfonamides, respectively. The recovery of the method was 63.7%～87.8% at 5～50 ng/ml addition concentration, and the detection limit was 5 ng/ml.

关 键 词：磺胺药物 肠衣 高效液相色谱 荧光检测器 

分 类 号：TS207.53[轻工技术与工程—食品科学]