太湖沉积物中解磷细菌分布及其与碱性磷酸酶活性的关系  

ALPase Activity and the Distribution of Phosphate Solubilizing Bacteria and the Relationship Between Them in Sediments of Lake Taihu

作　　者：宋炜[1] 袁丽娜[1] 肖琳[1] 詹忠[1] 杨柳燕[1] 蒋丽娟[1] 

机构地区：[1]南京大学环境学院污染物控制与资源化国家重点实验室,南京210093

出　　处：《环境科学》2007年第10期2355-2360,共6页Environmental Science

基　　金：国家自然科学基金项目(40371102);国家重点基础研究发展规划(973)项目(2002CB412307)

摘　　要：分析了太湖沉积物中解磷细菌的丰度,测定了不同季节、不同采样点、不同取样深度沉积物中碱性磷酸酶的活性,探讨了解磷细菌分布与碱性磷酸酶活性的关系.结果表明,解磷菌在表层沉积物中普遍存在,随着深度的增加,数量逐渐减少.解磷菌的种类秋季最多,冬季最少,但解磷能力较强的菌大部分出现在夏季.碱性磷酸酶活性范围为0.001～0.006mmol·（g·min）^-1,酶活性呈现较为明显的季节变动,不同采样点间酶活性差异较大,随深度的增加,碱性磷酸酶活性逐渐降低.各采样点碱性磷酸酶在0～12 cm活性较高且变化较大,12 cm以下深度酶活性较低,基本稳定在0.001 mmol·（g·min）^-1左右.沉积物中碱性磷酸酶活性与解磷菌数量有一定相关性,相关系数为0.50～0.85.The abundance of phosphate solubilizing bacteria, together with the alkaline phospbatase（ALPase） activity in the sediment samples of Lake Taihu in different seasons, sites and depths, was analyzed. And the relationship between the distribution of phosphate solubilizing bacteria and the ALPase activity was discussed. Results show that phosphate solubilizing bacteria is universally detected in surface sediments. With the increasing depth, the amount of phosphate solubilizing bacteria decreases. The number of phosphate solubilizing bacteria strains reaches the maximum in autumn while the minimum in winter. ALPase activity ranges from 0.001 mmol·（g·min）^-1 to 0.006 mmol·（g·min）^-1 and it varies with different sites and seasons and declines with the increasing depth. ALPase activity varies widely above 12cm, and stabilizes about 0.001 mmol·（g·min）^-1 under 12 cm in each site. There is a correlation between ALPase activity and the number of phosphate solubilizing bacteria and the correlation coefficients range from 0.50 to 0.85.

关 键 词：沉积物 微生物 磷释放 碱性磷酸酶 

分 类 号：X171[环境科学与工程—环境科学]