槐米总黄酮提取物的热分析指纹图谱  

Thermal Analysis Fingerprint of Flavonoids Extracted from Buds of Sophora japonica L.

作　　者：张黎明[1] 赵希[1] 张小利[1] 王玲玲[1] 

机构地区：[1]天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300222

出　　处：《应用化学》2007年第10期1206-1210,共5页Chinese Journal of Applied Chemistry

摘　　要：以芦丁标准品作对照,应用热重法(TG)和微商热重法(DTG)对不同提取方法(碱提酸沉法、乙醇回流法和超声辅助法)以及提取条件(碱提取液pH值、稳定剂种类)下制备的槐米总黄酮提取物进行热重分析及热特征图谱解析。结果表明,由于提取方法和条件不同,产品质量有明显区别,反映在TG-DTG曲线上,表现出不同峰形、峰位及失重情况。在碱提酸沉法提取中,碱提取液pH=9,硼砂作稳定剂时,提取物的产率和含量均较高,而且其TG-DTG曲线特征与芦丁标准品比较接近。因此这些热分析图谱可以作为控制槐米总黄酮提取物质量的指纹图谱。Flavonoids were extracted from the alkali dissolution of acid sedimentation, ethanol buds of Sophora japonica L. by different methods, such as reflux, and ultrasonic-assisted methods. The extraction conditions, including the pH of the base and the kind of stabilizer were researched as well. Furthermore, with rutin as a control, thermal characteristics of flavonoids from the buds of Sophora japonica L. were determined via the TG-DTG curves. The results showed that every sample had different mass loss, curve shape, and peak location related to different extraction technology. The TG-DTG characteristics of the products extracted by alkali dissolution of acid sedimentation with the extract adjusted to pH = 9 and borax as the stabilizer were similar to that of standard rutin. Meanwhile, the yield and purity of the products extracted by the above optimum extraction technology were higher than others. Therefore, the thermal analysis fingerprint could serve as a tool for quality control of flavonoids from the buds of Sophora japonica L.

关 键 词：槐米 黄酮类化合物 芦丁 热重分析谱图 

分 类 号：O641.2[理学—物理化学]