肠出血性大肠埃希菌O157∶H7黏附HeLa细胞模型的建立  

Establishment of an enterohemorrhagic Escherichia coli O157∶H7 attached HeLa cell model

作　　者：余抒[1] 顾江[1] 曾浩[1] 杨琴[1] 刘艳青[1] 张卫军[1] 罗萍[1] 王庆旭[2] 毛旭虎[1] 

机构地区：[1]第三军医大学医学检验系临床微生物及免疫学教研室,重庆400038 [2]成都军区总医院检验科,成都610000

出　　处：《第三军医大学学报》2007年第20期1933-1935,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30670107);国家高技术发展研究计划"863"项目(2006AA02Z405)~~

摘　　要：目的建立肠出血性大肠埃希菌O157∶H7体外黏附HeLa细胞模型,为进一步研究其致病机制奠定基础。方法以细菌黏附HeLa细胞数量为指标,分别评价细菌数量、细菌与细胞孵育时间对细菌黏附数量的影响,得到最佳条件,最后肌动蛋白荧光染色(FAS)试验鉴定模型。结果加入细菌为1×105CFU,细菌与细胞孵育4h时,黏附细胞的数量最佳,FAS检测发现该条件下细菌能够引起细胞特异性黏附、擦拭(attaching&effacing,A/E)损伤,模型建立成功。结论成功建立O157∶H7体外黏附HeLa细胞模型,为进一步研究其致病机制提供了条件。Objective To establish Enterohemorrhagic Escherichia coli （EHEC） O157：H7 attached HeLa cell model for further study on its pathogenic mechanism. Methods HeLa cells and Escherichia coli O157：H7 were incubated together in different condition （amount of bacteria, incubation time）. In regard to the amount of bacteria attached, we decided the best condition for O157：H7 to infect HeLa cells and identified the characterized A/E （ attaching and effacing） lesion by FAS （fluorescence actin staining）. Results The best condition is that 1 × 10^5 CFU O157：H7 and HeLa cells were incubated together for 4 h, under which the specific A/E lesion can be observed. Conclusion EHEC O157：H7 attached HeLa cell model has been successfully established.

关 键 词：肠出血性大肠埃希菌O157:H7 细胞模型 HELA细胞 

分 类 号：R-33[医药卫生] R378.21