Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules  

作　　者：王化斌 周星飞 安红杰 郭云昌 孙洁林 张益 胡钧 

机构地区：[1]Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800 [2]Department of Physics, Ningbo University, Ningbo 315211 [3]Tianjin University of Science and Technology, Tianjin 300040 [4]Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030 [5]Graduate School of the Chinese Academy of Sciences, Beijing 100080

出　　处：《Chinese Physics Letters》2007年第3期644-647,共4页中国物理快报（英文版）

基　　金：Supported by the National Natural Science Foundation of China under Grant Nos 10335070, 10404032, 30470441, 90406024-6, 60575029, 10304011 and 10604034, the Chinese Academy of Sciences under Grant No Y2005029, the Science and Technology Com- mission of Shanghai Municipality under Grant Nos 04qmx1466, 0552nm033 and 0552nm027, the Ningbo Municipal Natural Science Foundation of China under Grant No 2006A610046, and the New Energy and Industrial Technology Development 0rganization under Grant No 051IS128.

摘　　要：Effects of substrate hydrophobicity/hydrophilicity on height measurement of individual ds-DNA molecules are investigated with tapping mode atomic force microscopy （TMAFM） and vibrating mode scanning polarization force microscopy （VSPFM）. The measured heights of ds-DNA on hydrophobic highly oriented pyrolytic graphite （HOPG） are remarkably less than those on hydrophilic bare mica and Ni^2＋ treated mica in both TMAFM and VSPFM. By analysing the results, we propose that the hydrophobicity/hydrophilicity of substrate can greatly influence the height measurement of DNA molecules.Effects of substrate hydrophobicity/hydrophilicity on height measurement of individual ds-DNA molecules are investigated with tapping mode atomic force microscopy （TMAFM） and vibrating mode scanning polarization force microscopy （VSPFM）. The measured heights of ds-DNA on hydrophobic highly oriented pyrolytic graphite （HOPG） are remarkably less than those on hydrophilic bare mica and Ni^2＋ treated mica in both TMAFM and VSPFM. By analysing the results, we propose that the hydrophobicity/hydrophilicity of substrate can greatly influence the height measurement of DNA molecules.

关 键 词：ATOMIC-FORCE MICROSCOPY ADHESION FORCES PROTEIN ELASTICITY ADSORPTION PEPTIDE COMPLEX BINDING GROWTH WATER 

分 类 号：O59[理学—应用物理]