番茄叶黄素合成突变与光能分配和抗氧化酶活性的关系  

Relationship Between Lutein Deficit and Light Allocation as well as Antioxidants in Tomato

作　　者：王彦杰[1] 夏晓剑[1] 周艳虹[1] 喻景权[1] 

机构地区：[1]浙江大学园艺系,杭州310029

出　　处：《园艺学报》2007年第5期1301-1304,共4页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30671428;30500344);国家杰出青年科学基金项目(30235029)

摘　　要：以叶黄素(Lutein)缺失的番茄突变体(Xa)为材料,研究其光合和叶绿素荧光特性、吸收光能分配、细胞器水平抗氧化酶活性及抗氧化物质含量。结果表明:Xa突变体的非光化学猝灭系数(qN)、叶片吸收光能分配依赖于△pH和叶黄素的非结构性热耗散(J_(NPQ))显著低于其野生型Ailsa Craig,但净光合速率(Pn)、羧化效率(CE)、光化学猝灭系数(qP)与Ailsa Craig相似。此外,Xa突变体叶片细胞器水平的抗氧化酶活性和抗氧化物质含量大多数显著高于Ailsa Craig。这些结果表明,叶黄素缺失体主要通过降低光能吸收和提高抗氧化能力来避免过剩光能导致光氧化胁迫的产生。　A lutein deficit mutant（Xa）of tomato（Lycopersicon esculentum Mill.）was used to study the relationship between xanthophylls cycle and light allocation as well as antioxidant levels. Xa mutant showed significantly lower non-photochemical quenching（qN）,△pH and xanthophyll-regulated thermal energy dissipation（J（NPQ））than those of Ailsa Craig, but similar net photosynthesis rate（Pn）,carboxylation efficiency （CE）and photochemical quenching（qP）to those of Ailsa Craig. Moreover,Xa mutant exhibited increased activities of most antioxidant enzymes in cellular organelles and higher antioxidants contents in the leaves as compared to Ailsa Craig. All these results suggested that Xa mutant protected plant cells from photooxidation damage mainly by decreasing light adsorption and increasing antioxidant capacity.

关 键 词：番茄 突变体 非光化学猝灭 细胞器 光保护 叶黄素 

分 类 号：S641.2[农业科学—蔬菜学]