啤酒麦糟诱导里氏木霉生产纤维素酶的研究  

Production of Cellulase with Trichoderma reesei Induced by Beer Brewer's Spent Barley Grain

作　　者：刘超纲[1] 刘飞跃 余世袁[3] 

机构地区：[1]中南林学院林工系,株洲421006 [2]株洲啤酒厂,株洲412001 [3]南京林业大学,南京210037

出　　处：《中南林学院学报》1997年第1期42-45,共4页Journal of Central South Forestry University

摘　　要：以里氏木霉（Trichodermareesei）RutC30为产酶菌株．经适当预处理后的啤酒麦糟为碳源或诱导物，通过深层培养可获得较高浓度的纤维素酯液．当固体碳源浓度为20g／L,碳氮比为8．5时，于初始pH＝4.8，温度26～28℃，转速150r／min下培养，其发酵时间为6d．酶液浓度可达3．8FPIU／ml，酶产率为172FPIU／g纤维素.High-concentration cellulase liquid can be obtained by using 7richoderma reesei Rut C30 as the cellulase-producing strains and the beer brewer's spent barley grain pretreated as the carbon sources or inducers. Under the conditions that the mass concentration of solid carbon sources is 20 g/L, the ratio of carbon to nitrogen is 17: 2, inital pH is 4. 8, temperature is in 26～28℃ and rotational speed is 150 r/min, the cellulase-producing strains are cultivated. After 6-day's fermentation, the mass concentration of cellulase liquid reaches 3. 8 FPIU/mL, and the output of cellulase is 172 FPIU/g(cellulase).

关 键 词：纤维素酶 诱导 里氏木霉 啤酒麦槽 

分 类 号：TQ925.9[轻工技术与工程—发酵工程] TS261.9