蛋白质电泳在杂交水稻种子纯度鉴定中的应用  

Application of protein electrophoresis on the seed identification of hybrid rice

作　　者：张凤[1] 李鹏[1] 许鸿川[1] 祁建民[1] 陈伟[1] 

机构地区：[1]福建农林大学生命科学学院

出　　处：《福建农业学报》2007年第3期235-240,共6页Fujian Journal of Agricultural Sciences

基　　金：福建省重点科技项目(2003N032)

摘　　要：以4个三系配套杂交水稻组合及7个杂交稻F1代种子为试验材料,提取其醇溶蛋白和盐溶蛋白,利用SDS-PAGE技术进行图谱分析。结果表明:醇溶蛋白的多态性较弱,差异不大,蛋白质特征带不明显,不适合用于杂交水稻组合和杂交种子的真实性和纯度的检测;而盐溶蛋白的多态性丰富,差异明显,蛋白质特征带稳定,杂交水稻种子蛋白质电泳鉴定与田间小区种植鉴定结果基本吻合。SDS-PAGE技术简便快捷、分离效果好,是一种经济有效的技术,可作为杂交水稻F1代种子纯度蛋白质指纹图谱鉴定。Salt- and alcohol-soluble proteins extracted from the seeds of four different hybrid rice and their three lines, as well as seven other F1 hybrid progenies were analyzed by means of SDS- PAGE. The results showed that the polymorphism of alcohol-soluble proteins was not significant enough for authenticity and purity assessment. However, the salt-soluble proteins had a high polymorphism with stable and specific bands. The experiments demonstrated that SDS- PAGE was a simple, rapid, effective and inexpensive method for the identification of hybrid rice seeds.

关 键 词：杂交水稻 种子 醇溶蛋白 盐溶蛋白 SDS—PAGE纯度鉴定 

分 类 号：Q512[生物学—生物化学] S511[农业科学—作物学]