茶多酚对肺癌SPC-A1细胞增殖的影响  

Effects of tea polyphenols on proliferation of lung cancer cell line SPC-A1

作　　者：杜春华[1] 成炜[1] 胡晓玲[1] 王美[1] 秦筱梅[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003

出　　处：《山东医药》2007年第31期1-3,共3页Shandong Medical Journal

基　　金：山东省卫生厅科技发展计划项目(2001CACKB1)

摘　　要：目的探讨茶多酚对人肺癌SPC—Al细胞增殖的影响。方法体外培养人肺腺癌SPC—A1细胞，以50—300mg／L茶多酚作用12、24、36h后，MTT法测定细胞的增殖活性，琼脂糖凝胶电泳、透射电镜观察细胞凋亡情况，流式细胞仪检测对细胞周期的阻滞作用。结果茶多酚可抑制肺癌细胞的增殖活性，其抑制作用随时间的延长而增强，以36h抑制作用最强。浓度50～150mg／L，抑制作用随浓度增高而增强；浓度〉150mg／L，抑制作用随浓度增高反而减弱。茶多酚可使肺癌细胞阻滞于G1期，以150mg／L阻滞作用24h最强。结论茶多酚可诱导人肺癌SPC-Al细胞的凋亡，并使细胞周期阻滞于G1期，抑制肺癌细胞的增殖。[ Objective] To study the effects of tea polyphenols（TP）on proliferation of lung cancer cell line SPC-Al. [ Methods] SPC-Al cell line was cultured in vitro and treated with TP at 50-300 mg/L for 12, 24 and 36 h. Cell proliferation was evaluated by MTT, apoptosis was observed by gel electrophoresis and electric microscope, blockage of cell cycle was detected by flow cytometry. [ Results ] The proliferation of SPC-Al was significantly inhibited by TP and the inhibition rate was time-dependent（ 12-36 h） and concentration-dependent（50-150 mg/L）. The inhibitory effect decreased when the concentration exceeded 150 mg/L. SPC-A1 cells were blocked in GI phaes by TP. The highest G1 peak was detected at the concentration of 150 mg/L after 24 h. [ Conclusion] TP can inhibit the proliferation of SPC-Al cells in vitro by inducing cancer cell apoptosis and blocking cell cycle.

关 键 词：茶多酚 肺肿瘤 细胞凋亡 细胞周期 

分 类 号：R734.2[医药卫生—肿瘤]