离子交换树脂提取发酵液中聚-ε-赖氨酸的研究  

Extraction of poly-ε-lysine from fermentation broth by ion-exchange process

作　　者：陈雄[1] 袁金凤[1] 王实玉[1] 章莹[1] 王金华[1] 

机构地区：[1]湖北工业大学生物工程学院,湖北省工业微生物重点实验室,武汉430068

出　　处：《食品科技》2007年第10期144-146,共3页Food Science and Technology

基　　金：湖北省教育厅青年项目(Q200514001)。

摘　　要：对离子交换树脂提取分离发酵液中聚-ε-赖氨酸(ε-PL)的工艺条件进行研究,确立的最佳提取条件为使用弱酸性阳离子交换树脂D152,上柱液pH8.5,以盐酸作为洗脱剂,洗脱剂浓度为0.1mol/L,洗脱速度为1.0mL/min。在优化条件下,ε-PL平均提取得率为82%,产品纯度达到了80%以上。The study was conducted to explore the optimum conditions of extraction and isolation of poly-ε-lysine （ε-PL） from fermentation broth. It was founded that the optimum conditions were listed as follow： ε-PL was isolated by using weak acidic cation exchange resin D152 with the pH of fermentation broth before it was put into the resin column being adjusted to 8.5, HCI elution with 0.1mol/L and elution speed at 1.0mL/min. Applying the above conditions,ε-PL was yielded at a rate about 82% and the content of ε-PL is more than 80%.

关 键 词：聚-ε-赖氨酸 发酵液 提取 离子交换 

分 类 号：TQ92[轻工技术与工程—发酵工程]