Two-dimensional Electrophoresis Analysis of Proteins Extracted from Alexandrium sp. LC3  

作　　者：LI Hao MIAO Jinlai CUI Fengxia LI Guangyou 

机构地区：[1]College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China [2]Key Laboratory of Marine Biological Active Substances, SOA, Qingdao 266061, P. R. China [3]College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P. R. China

出　　处：《Journal of Ocean University of China》2007年第4期365-368,共4页中国海洋大学学报（英文版）

基　　金：This research was financially supported by National Natural Science Foundation of China （40206022）.

摘　　要：Two-dimensional electrophoresis（2-DE） of protein extracted and purified from Alexandrium sp. LC3 was conducted. In the SDS-PAGE study, the relative molecular weights of the proteins were mainly in the range of 14 kDa-31 kDa and 43 kDa-66 kDa, and more proteins were detected between 14kDa and 31 kDa. With the improved protein preparation, the two-dimensional electrophoresis patterns indicated that the relative molecular weights of the proteins were between 14kDa and 100kDa, and most of them ranged from 14 kDa to 31 kDa. This was consistent with the result of the SDS-PAGE analysis. The isoelectric points were found to lie between 3.0 and 8.0, and most of them were in the range of 3.0-6.0. Better separation effect was acquired with pre-prepared immobilized gradient （IPG） strip （pH 3-5.6）, and about 320 protein spots could be visualized on the 2-DE map by staining. Within pH 3-l0 and pH 3-5.6 strips, the protein samples of Alexandrium sp. LC3 could be separated well.Two-dimensional electrophoresis(2-DE) of protein extracted and purified from Alexandrium sp. LC3 was conducted. In the SDS-PAGE study, the relative molecular weights of the proteins were mainly in the range of 14 kDa?31 kDa and 43 kDa?66 kDa, and more proteins were detected between 14 kDa and 31 kDa. With the improved protein preparation, the two-dimensional electrophoresis patterns indicated that the relative molecular weights of the proteins were between 14 kDa and 100 kDa, and most of them ranged from 14 kDa to 31 kDa. This was consistent with the result of the SDS-PAGE analysis. The isoelectric points were found to lie between 3.0 and 8.0, and most of them were in the range of 3.0?6.0. Better separation effect was acquired with pre-prepared immobilized gradient (IPG) strip (pH 3?5.6), and about 320 protein spots could be visualized on the 2-DE map by staining. Within pH 3?10 and pH 3?5.6 strips, the protein samples of Alexandrium sp. LC3 could be separated well.

关 键 词：Alexandrium sp. LC3 PROTEIN SDS-PAGE two-dimensional electrophoresis 

分 类 号：P73[天文地球—海洋科学]