Bcl-2反义寡核苷酸与Rituximab联合对Raji细胞增殖和凋亡的影响  

Combinational effects of bcl-2 antisense oligodeoxynucleotide and Rituximab on proliferation and apoptosis of B-lymphoma Raji cells

作　　者：申咏梅[1] 杨晓春[1] 石怡珍[1] 谢小芳[2] 唐军[1] 

机构地区：[1]苏州大学附属第二医院放免中心,江苏苏州215004 [2]苏州大学附属第二医院检验教研室,江苏苏州215004

出　　处：《细胞与分子免疫学杂志》2007年第8期727-730,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30400111);江苏省自然科学基金资助项目(BK2004041)

摘　　要：目的：研究bcl-2硫代反义寡核苷酸（bcl-2 ASODN）联合Rituximab[CD20单克隆抗体（mAb）]对B细胞淋巴瘤Raji细胞株体内外增殖和凋亡的影响, 并探讨其作用机制.方法：bcl-2 ASODN和Rituximab分别和联合作用B细胞淋巴瘤Raji细胞后, 通过MTT法检测细胞生长情况;流式细胞术（FCM）检测bcl-2蛋白表达和细胞凋亡;RT-PCR检测bcl-2 mRNA表达水平;用Raji细胞建立裸鼠B细胞淋巴瘤模型观察bcl-2 ASODN与Rituximab联合在体内的抗肿瘤效果. 结果：5～30 μmol/L bcl-2 和1 ～16 mg/L Rituximab单独应用均能抑制Raji细胞生长、诱导细胞凋亡、使bcl-2蛋白和bcl-2 mRNA表达降低, 但两者联合应用较单独应用抑制作用更为明显（P＜0.01）.体内实验表明, bcl-2 ASODN与Rituximab联合应用较单独可有效抑制BALB/c裸鼠B细胞淋巴瘤的生长（P＜0.01）.结论：bcl-2 ASODN联合Rituximab较分别单独应用可明显抑制B细胞淋巴瘤Raji细胞生长, 促进细胞凋亡;其机制可能是通过联合下调bcl-2蛋白及bcl-2 mRNA表达而起作用.AIM： To study the combinational effects of bcl-2 antisense oligodeoxynucleotide （bcl-2 ASODN ） and Rituximab （anti-CD20 monoclonal antibody） on proliferation and apoptosis of B-lymphoma Raji cells in vitro and in vivo, and to explore the possible mechanisms. METHODS ： After bcl-2 ASODN and Rituximab treating on Raji cells respectively or cooperatively, the proliferation of Raji cells, the expression of bcl-2 protein, the apoptosis and the expression of bcl-2 mRNA were detected by MrT assay, flow cytometry （FCM） and RT-PCR, respectively. The combinational antitumor activity of bcl-2 ASODN and Rituximab was evaluated in BALB/c nude mice bearing B-lymphoma inoculated with Raji cells. RESULTS： 5 -30 μmol/L bcl-2 ASODN and 1 - 16 mg/L Rituximab could inhibit Raji cells＇ growth, induce apoptosis and decrease the expression of bcl-2 protein and bcl-2 mRNA alone. But there was much more effective inhi- bition of growth and induction of apoptosis by their combination than alone （ P 〈 0.01 ）. In tumor bearing mice, combination of bcl-2 ASODN and Rituximab showed a significant inhibitory effect on the growth of transplanted B-lymphoma, resulting in a statistically significant difference compared with alone （P 〈 0.01 ）. CONCLUSION： It has been found that bcl-2 ASODN combined with Rituximab could significantly inhibit the growth of Raji cells and induce their apoptosis via reinforcing specific down regulation of bcl-2 protein and bcl-2 mRNA expression.

关 键 词：BCL-2 反义寡核苷酸 RITUXIMAB Raji 

分 类 号：Q392[生物学—遗传学]