金线鱼肝胰脏蛋白酶激活提取条件的优化  

Optimization of activation and extraction of protease in hepatopancreas from Nemipterus virgatus (Houttuyn)

作　　者：陶红[1] 赵谋明[1] 崔春[1] 

机构地区：[1]华南理工大学轻工与食品学院

出　　处：《吉林大学学报（工学版）》2007年第4期971-975,共5页Journal of Jilin University:Engineering and Technology Edition

基　　金：广东省科技计划项目(2003B21304);广东省农业攻关项目(2004A20302004)

摘　　要：用响应面法对金线鱼肝胰脏蛋白酶的激活及提取条件进行了优化。比较了不同激活剂的激活效果。研究了提取时间和pH值交互作用对蛋白酶活力的影响。结果表明:肠提液与CaCl2协同作为激活剂的激活效果最好。优化后激活时间为6 h,激活温度为27℃,肠提液质量分数为7%,CaCl2浓度为5.20 mmol/L,酶活力为1578.14 U/(g.min)。肝胰脏蛋白酶最佳提取条件为:提取时间8.5 h,提取温度11℃,pH 5.4,酶活力为1431 U/(g.min)。蛋白酶复性电泳结果说明酶活力增加显著。The activation and extraction conditions of protease in hepatopancreas from Nemipterus virgatus （Houttuyn） were optimized by response surface methodology （RSM）. The activation effects of different activators were compared. The influence of the interaction between extraction time and pH value on protease activity was studied. Results show that the obtained protease activity is up to 1578.14 U/（g · min） under the optimum activation conditions of temperature 27 ℃, time 6 hours, intestine extract concentration 7%, CaC12 concentration 5. 20 mmol/L. The optimum extraction conditions are temperature 23 ℃, time 8.5 hours and pH 5.4,can obtain a protease activity of 1431 U/（g · min）. Result of non-denatured electrophoresis indicates that the protease activity is increased evidently.

关 键 词：食品加工技术 蛋白酶 响应面方法 激活 提取 复性电泳 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程] TS201.2[轻工技术与工程—食品科学与工程]