山杏种皮黑色素的抗氧化活性  

Antioxidant Properties of Melanin from Testae of Armeniaca vulgaris var. ansu

作　　者：姚增玉[1] 李科友[1] 赵忠[1] 马希汉[1] 

机构地区：[1]西北农林科技大学林学院

出　　处：《林业科学》2007年第10期59-63,共5页Scientia Silvae Sinicae

基　　金：国家林业局"948"项目(2004-4-52)

摘　　要：对山杏种皮黑色素的抗氧化活性进行评价。结果表明:山杏种皮黑色素可以显著抑制β-胡萝卜素的氧化褪色,其清除自由基活性具有量效性,清除羟自由基能力与2,6-二叔丁基对甲酚(BHT)无显著差异而高于抗坏血酸(VC),清除超氧阴离子能力超过BHT和VC。山杏种皮黑色素既可以作为氢供体,清除DPPH自由基,但清除能力低于BHT和VC,也可作为电子供体,其还原能力高于BHT和VC。The antioxidant properties of the melanin extracted from testae of Armeniaca vulgaris var. ansu were investigated with various methods： β-carotene-linoleate model system, scavenging of radicals such as hydroxyl radical （· OH）, superoxide anion （O2^- ） and 2, 2-diphenyl-l-picrylhydrazyl （DPPH·）. The reducing power and the total phenolic content of the melanin also were determined. The melanin strongly inhibited β-carotene oxidation and exhibited dose-dependent free-radical-scavenging activities against hydroxyl radicals, superoxide anions and DPPH radicals. The melanin was also found to have excellent reducing power. The reducing power of the melanin was higher than that of ascorbic acid （VC） and butylated hydroxytotuene （BHT） at 100 ～ 500 μg·mL^- 1 indicating that the melanin from testae of A. vulgaris var. ansu had a strong electron-donating capacity. These antioxidant properties of the melanin opened up a vast range of prospects for making use in many fields, especially in functional food industry and pharmacy.

关 键 词：黑色素 山杏 抗氧化 还原力 自由基 

分 类 号：S718.43[农业科学—林学]