低分子肝素相对分子质量与抗凝活性关系研究  

Study on relationship between molecular weight and anticoagulant effect of low molecular weight heparins

作　　者：李京[1] 范慧红[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《中国新药杂志》2007年第20期1695-1697,1701,共4页Chinese Journal of New Drugs

基　　金：北京市自然基金(7032041)

摘　　要：目的:研究低分子肝素相对分子质量与抗凝活性之间的关系。方法:用生色底物法测定不同相对分子质量的低分子肝素6个分级和未分级样品的抗Ⅹa因子和抗Ⅱa因子效价,相对分子质量采用高效体积排阻色谱法(high performance size exclusion chromatography,HPSEC)测定。结果:抗Ⅹa因子与抗Ⅱa因子效价比随着相对分子质量的降低而增大。结论:在测定范围内,低分子肝素相对分子质量降低,抗凝活性降低,抗栓作用增强。Objective：To investigate the relationship between molecular weight and anticoagulant effect of low molecular weight heparins. Methods：The potency of anti-factor Xa and anti-factor Ⅱa in six fractionated and unfractionated samples of low molecular weight heparins with different molecular Weights were determined by chromogenic substrate method. Molecular weights of samples were determined by high performance size exclusion chromatography. Results：The potency ratio of anti-factor Ⅹa to anti-factor Ⅱa was increased with the decrease of molecular weight. Conclusion： In the studied range, with the decrease of molecular weight, the anticoagulant effect of low molecular weight heparins is decreased, the antithrombotic effect is increased.

关 键 词：低分子肝素 相对分子质量 抗凝活性 生色底物法 

分 类 号：R972.7[医药卫生—药品] R965.1[医药卫生—药学]