稻米淀粉黏滞性(RVA谱)特征值的遗传及QTL定位分析  

Analysis of Inheritance and QTLs of Rice Starch Viscosity (RVA Profile) Characteristics

作　　者：张巧凤[1] 张亚东[1] 朱镇[1] 赵凌[1] 赵庆勇[1] 许凌[1] 王才林[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心

出　　处：《中国水稻科学》2007年第6期591-598,共8页Chinese Journal of Rice Science

基　　金：农业部超级稻新品种选育与示范项目;农业结构调整重大技术研究专项资助项目(05-01-05B);江苏省高技术研究项目(BG2004304;BG2005301)

摘　　要：利用武育粳3号/Aichi106构建的F2群体,研究了精米粉RVA谱8个特征值:最高黏度(peak viscosity,PKV)、热浆黏度(hot paste viscosity,HPV)、冷胶黏度(cool paste viscosity,CPV)、崩解值(breakdown viscosity,BDV)、消减值(setback viscosity,SBV)和回复值(consistence viscosity,CSV)、峰值时间(peak ti me,PeT)、糊化温度(pasting tempera-ture,PaT)的遗传规律。结果表明,PKV为由微效基因控制的数量性状;HPV、CPV、SBV、BDV、CSV可能是由一对主基因和微效多基因共同控制的数量性状;PeT可能由两对主基因控制,并且还受微效多基因的影响。用热研2号/密阳23构建重组自交系群体,于2005-2006年对稻米淀粉RVA谱8个特征值进行了数量性状基因座QTL定位和分析,两年试验共检测到34个QTL,分布在第1、2、3、4、6、7、8共7条染色体上;两年同时检测到的QTL共8个,其中qHPV6、qCPV6、qCSV6、qSBV6、qBDV6位于第6染色体的Wx基因附近;qHPV2、qCSV2位于第2染色体RM341与RM475标记之间,qCPV2位于RM573与RM250标记之间。第2染色体上与支链淀粉合成有关的两个基因与RVA谱特征值有密切关系。The rice starch viscosity （RVA profile） characteristics has been proved useful for the evaluation of eating and cooking quality in rice breeding program. To study the inheritance of the RVA profile, an F2 population of Wuyujing 3/Aichi 106 was used. The results indicated that peak viscosity （PKV） was a typical quantitative character; hot paste viscosity （ HPV）, cool paste viscosity （CPV）, setback viscosity （SBV）, breakdown viscosity （BDV）, consistence viscosity （CSV） might he controlled by a major gene and several minor genes; and peak time （PET） might he controlled by two major genes and several minor genes. In order to understand the genetic basis of the paste viscosity characteristics, the recombinant inbred line populations derived from Nikken 2/Milyang 23 and its genetic linkage map were used to map the QTLs controlling RVA profiles in 2005 and 2006. The results showed a total of 34 QTLs distributed on chromosomes 1, 2, 3, 4, 6, 7 and 8 in 2005 and 2006, including 19 and 15 QTLs in 2005 and 2006, respectively; eight QTLs were both found in two years, the qHPV6, qCPV6, qCSV6, qSBV6, qBDV6 were located on chromosome 6, while qHPV2, qCSV2, qCPV2 were located on chromo- some 2. The genes involved in amylopectin synthesis on chromosome 2 were greatly related with RVA profile.

关 键 词：淀粉黏滞性谱 重组自交系群体 遗传 数量性状座位 稻米 淀粉 

分 类 号：S511[农业科学—作物学]