原发性肝癌患者乙型肝炎病毒核心启动因子变异与IL-10、IL-12、TNF-α及IFN-γ关系的探讨  

Relationship between HBV BCP mutation and serum IL-10, IL-12, TNF-α as well as IFN-γ in HCC

作　　者：黄力毅[1] 宣伟军[2] 玉艳红[1] 吴继周[1] 

机构地区：[1]广西医科大学第一附属医院感染性疾病科,广西南宁530021 [2]广西中医学院,广西南宁530001

出　　处：《中国现代医学杂志》2007年第20期2488-2491,共4页China Journal of Modern Medicine

基　　金：广西科学基金项目(桂科自0339050);广西卫生厅课题(桂卫Z2004127)

摘　　要：目的研究原发性肝癌患者乙型肝炎病毒核心基因启动子变异(HBVBCP)与IL-10、IL-12、TNF-α及IFN-γ关系。方法176例HBV慢性感染者[慢性乙型病毒性肝炎轻、中、重度,肝炎肝硬化,慢性重型肝炎和原发性肝癌(HCC)]。采用PCR微板核酸杂交结合ELISA检测显示技术,对患者血清进行检测HBVBCP区核苷酸(nt)1762碱基A→T和1764G→A联合突变;采用双抗体夹心ELISA检测技术,检测患者血清细胞因子(IL-10、IL-12、TNF-α和IFN-γ)水平。结果HBVBCP变异在HCC组的阳性率为70.0%(14/20)显著高于慢性肝病组的阳性率37.8%(59/156)(P=0.008);HCC组的血清TNF-α水平明显高于慢性肝病组(P=0.000),而HCC组的血清IL-10、IL-12和IFN-γ水平与慢性肝病组的比较差异无显著性(P>0.05)。结论HBVBCP变异与原发性肝癌关系密切,且血清TNF-α在原发性肝癌的发生中可能起到一定的作用。[Objective] To investigate the relationship between hepatitis B virus （HBV） basic core promoter （BCP） mutation and serum IL-10, IL-12, TNF-α as well as IFN-γ, in hepatocellular carcinoma （HCC） patients. [Methotis] Project subject： 176 patients （Chronic hepatitis B with mild, moderate and sever; hver cirrhosis, Chronic fulminant and HCC） with chronic hepatitis B virus infection were studied. Project methods： The A to T mutation at nucleotide 1762 and G to A mutation at nucleotide 1764 were determined by the method of polymerase chain reaction （PCR） microplate hybridization ELISA in these patients. The serum cytokines（IL-10, IL-12, TNF-α and IFN-γ,） of these patients were measured by specific-ELISA. [Results] The positive rate of HCC was significantly higher than that of chronic liver disease （P =0.008）. The serum level of TNF-α in HCC was significantly higher than that of chronic liver disease （P =0.000）. There was no significant difference between the groups of HCC and that chronic liver disease group in the serum levels of IL-10 and IL-12 as well as IFN-γ, （P 〉0.05）. [Conclusion] HCC correlated with HBV BCP mutants. There was a hight positive rate of HBV BCP mutants in the HCC. The serum levels of TNF-α might play a major role on the development of HCC.

关 键 词：HBV BCP 基因变异 原发性肝癌 

分 类 号：R735.7[医药卫生—肿瘤]