细菌视紫红质(BR)的表面光电压谱研究  

SURFACE PHOTOVOLTAIC SPECTROSCOPIC INVESTIGATION OF BACTERIORHODOPSIN(BR)

作　　者：王宝辉[1,2] 王今堆[1,2] 傅学奇 王德军[3] 崔毅[3] 李铁津 

机构地区：[1]大庆石油学院石油化工系 [2]吉林大学分子生物系 [3]吉林大学化学系

出　　处：《光谱学与光谱分析》1997年第2期35-40,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金

摘　　要：采用表面光电压谱系统地研究了细菌视紫红质（ＢＲ）的光伏特性，结果表明ＢＲ薄膜有着独特的光电性质，并且与ＢＲ薄膜的制备方法和湿度有关。从经过ＨＥＰＥＳ修饰的ＢＲ表面光电压谱，观察到了ＢＲ的中间体Ｍ４１２和Ｏ６４０响应峰，说明ＨＥＰＥＳ对ＢＲ中间体的稳定作用。The surface photovoltaic spectra(SPS) of Bacteriorhodopsin based films were systematically measured. The results show that the BR films are characterized by the specific photovoltaic effect. The SPS response of the BR films depends on the fabrication and humidity of the film. The intermediate M 412 and O 640 of BR are observed in the SPS of HEPES containing BR film,indicating that HEPES has very important function to stabilize intermediate M 412 . The observations are explained in terms of the photocycle of BR and the mechanism of photovoltage generation.

关 键 词：细菌视紫红质 表面光电压谱 光电响应 

分 类 号：Q939.107[生物学—微生物学]