纳米氧化钛薄膜对颅成骨细胞黏附、增殖的影响  

Influence of nanophase titania films on skull osteoblast adhesion and proliferation

作　　者：贺捷[1] 陈万涛[1] 万澎波[1] 周晓健[1] 周威[2] 钟晓霞[2] 

机构地区：[1]上海交通大学附属第九人民医院口腔颌面外科,上海市口腔医学研究所,上海200011 [2]上海交通大学物理系,上海200030

出　　处：《口腔医学》2007年第9期449-451,465,共4页Stomatology

基　　金：国家自然科学基金项目(30572053);上海市纳米科技专项(0652nm018;0452nm063);上海市重点(优势)学科资助(Y0203)

摘　　要：目的为寻找牙种植体表面修饰的有效方法,本研究旨在探讨纳米氧化钛薄膜对颅骨成骨细胞黏附、增殖的影响。方法采用磁控溅射方法将纳米氧化钛薄膜溅射于抛光钛板上;通过原子力显微镜观测氧化钛薄膜表面形貌及粗糙度,并由图像分析软件测定;表面亲水性由接触角测量计测量;颅骨成骨细胞接种在材料表面,通过荧光显微镜、图像分析等方法体外研究细胞黏附、增殖情况;结果应用SPSS 11.5软件统计分析。结果磁控溅射方法能在抛光钛板表面溅射一层纳米氧化钛薄膜。当纳米氧化钛薄膜表面粗糙度及亲水性处于最适值时,细胞黏附、增殖最好;纳米氧化钛薄膜组细胞增殖显著优于未镀膜组。结论表面粗糙度高及亲水性较好的纳米尺度材料能促进细胞的黏附、增殖;对钛材料表面进行纳米尺度的改性是一种有前景的种植体表面修饰方法。Objective To find the effective method for modifying implant surface, and to investigate the influence of nanophase titania films on skull osteoblast adhesion and proliferation. Methods Titania films with surface structure of nanometer scale were deposited by reactive magnetron. The topography of the films was observed by atomic force microscope, the surface roughness was calculated by the image analysis software, and the wettability of surface was measured by contact angle meter. Rat skull osteoblast was cultured on different samples, and the surface roughness was calculated by the image to evaluate the biocompatibility of the materials. The cellular morphology was observed by fluorescence microscope. The number of cells was counted by image analysis software. SPSS 11.5 software was used for statistics analysis. Results The films deposited by the magnetron sputtering were nanophase titania. The films with smooth surface have hydrophilic surface property. The nanophase titania films can enhance skull osteoblast adhesion and proliferation. When the surface energy and surface roughness were at the optimum value, the cell adhesion and proliferation would be improved significantly. Conclusion The rough and hydrophihc nanophase surface can improve osteoblast adhesion and proliferation. This study provides a potential application of reactive plasma technology to improve the surface biocompatibility as dental and orthopedic implants.

关 键 词：纳米氧化钛 磁控溅射 成骨细胞 黏附 增殖 

分 类 号：R783.1[医药卫生—口腔医学]