固定化工程菌对偶氮染料脱色及强化作用  

Decoloration and Bioaugmentation on Azo Dye by Immobilized Genetically Engineered Strain

作　　者：金若菲[1] 周集体[1] 王竞[1] 曹同川[1] 

机构地区：[1]大连理工大学环境与生命学院,大连116024

出　　处：《环境科学》2007年第11期2598-2602,共5页Environmental Science

基　　金：辽宁省自然科学基金项目(20032122)

摘　　要：利用聚亚胺酯大孔泡沫吸附固定基因工程菌Escherichia coliJM109(pGEX-AZR),研究其对偶氮染料的脱色动力学及生物强化作用.实验表明,固定的E.coliJM109(pGEX-AZR)对酸性大红GR的脱色动力学符合Andrews方程,动力学常数为μmax,c、Kc、Kic分别为49.2 mg.(g.h)-17、10.43 mg.L-1和681.62 mg.L-1,R2为0.995.将固定的E.coliJM109(pGEX-AZR)按10%的比例投加到厌氧序批式活性污泥反应器中连续运行32 d,含有固定化工程菌的强化体系耐浓度冲击的能力和脱色率均高于对照体系,脱色率可以稳定在90%以上.利用RISA对其微生物群落结构进行分析,E.coliJM109(pGEX-AZR)及降解酸性大红GR的优势菌群可以在污泥体系中稳定存在.Decoloration and bioaugmentation on azo dye are investigated by using immobilized genetically engineered strain Escherichia coli JM109 （pGEX-AZR） on marcroporous foam carriers. The kinetics of the acid red GR decolorization by the immobilized E. coli JM109 （pGEX- AZR） accords with Andrews model proved by our experiments, and the kinetic parameters,μmax-c and Kc,Kic are found tO be 49.2 mg·（g·h）^-1, 710.43 mg·L^-1 and 681.62 mg·L^-1 respectively. For continuous operating in the anaerobic SBRs with 10% inoculation of Escherichia coli JM109 （pGEX-AZR） on marcroperous foam carriers for 32 d, both the tolerance to red GR concentration shock and the colorific removal in the bioaugmented anaerobic SBRs are higher than the control system, and the acid red GR decoloration rate reached 90%. Changes in microbial community have been detected by the RISA, in which the introduced immobilized GEM and preponderant mixed culture were subsisted steadily in sludge systems.

关 键 词：基因工程菌 固定化细胞 偶氮染料 动力学 生物强化 RISA 

分 类 号：X172[环境科学与工程—环境科学]