HPLC内标法同时测定食品中7种防腐剂的含量  

Determination of seven preservatives in foods by HPLC internal standard method

作　　者：王静[1] 白璐[1] 谷艳[1] 项婧[1] 赵春杰[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2007年第11期695-699,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的选择桂皮酸作为内标，建立HPLC法测定食品中山梨酸、苯甲酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸异丙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯。方法采用Dia—monsil（迪马）ODS（250mm×4．6mm，5μm）色谱柱，以乙腈、水（磷酸调pH：3）、四氢呋哺为流动相采用梯度洗脱，检测波长：254nm，流速：1．0mL·min^-1，样品用无水乙醇-水（体积比为85：15，磷酸调pH：2．5）提取。结果在上述条件下7种防腐剂可以达到基线分离，各组分对照曲线的线性相关性均大于0．9996，加样回收率：93．3％～100．5％，检出限：0．025～0．500gg。结论该方法适合食品中防腐剂的实际分析。Objective To develop a HPLC method for the determination of the contents of preservatives in cosmetic taking cinnamic acid as an internal standard. Therefore, the contents of sorbic acid, benzoic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, isopropyl p-hydroxybenzoate, propyl p-hydroxybenzoate and butyl p-hydroxybenzoate were evaluated. Methods The column was Diamonsil （Dikma） ODS （250.0 mm × 4.6 mm, 5μm） with acetonitrile, water（phosphate adjusted pH = 3） and tetrahydrofuran as the mobile phase. The way of gradient elution was adapted. The detection wavelength was set at 254 nm, The flow rate was 1.0 mL· min^- 1. Samples were extracted by ethanol-water （ V ： V = 85 ： 15, phosphate adjusted pH = 2.5）. Results Under this condition, the peaks of seven preservatives were separated, the linear correlation coefficient was no less than 0. 999 6, the recoveries were 93.3% - 100.5%, detection limits were 0. 025 - 0. 500 μg. Conclusions This method is simple, accurate and sensitive. It can be applied for food analysis.

关 键 词：高效液相色谱法 食品 防腐剂 内标法 

分 类 号：O657.72[理学—分析化学]