Construction and identification of immortalized rat astrocyte cell line expressing enkephalin  

作　　者：徐颖 田玉科 田学愎 安珂 场辉 

机构地区：[1]Department of Anesthesiology Children's Hospital of Chongqing University of Medical Sciences [2]Department of Anesthesiology Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology

出　　处：《Chinese Journal of Traumatology》2007年第6期353-356,共4页中华创伤杂志（英文版）

基　　金：the National Natural Science Foundation of China(No.30170905)and Paul Janssen Foundation.

摘　　要：Objective:To provide a sound cell source for further ex-vivo gene therapy for chronic pain,we attempt to develop an immortalized rat astrocyte cell line that expresses enkephalin regulated by doxycycline. Methods:Retrovirus infection method was employed to develop an immortalized rat astrocyte cell line that could express enkephalin regulated by doxycycline.The hPPE gene expression level of immoralized astroyte cells(IAC)/ hPPE was detected by RT-PCR,indirect immunofluorescence staining and radioimmunoassay. Results:IAC carrying Tet-on system transfected with preproenkephalin gene could secrete enkephalin that was regulated by doxycycline in a dose-dependent manner and hPPE gene activation could be repeated in on-off-on cycles through administration or removal of doxycycline. Conclusion:An immortalized rat astrocyte cell line that secrete enkephalin under the control of doxycycline is established successfully,which provides a research basis for transgenic cell transplantation for analgesia.

关 键 词：ENKEPHALINS Gene therapy ASTROCYTES Spinal cord injuries 

分 类 号：R614[医药卫生—麻醉学]